A compendium of DNA-binding specificities of transcription factors in Pseudomonas syringae

Ligang Fan, Tingting Wang, Canfeng Hua, Wenju Sun, Xiaoyu Li, Lucas Grunwald, Jingui Liu, Nan Wu, Xiaolong Shao, Yimeng Yin, Jian Yan () and Xin Deng ()
Additional contact information
Ligang Fan: Northwest University
Tingting Wang: City University of Hong Kong, Kowloon Tong
Canfeng Hua: City University of Hong Kong, Kowloon Tong
Wenju Sun: Northwest University
Xiaoyu Li: Northwest University
Lucas Grunwald: City University of Hong Kong, Kowloon Tong
Jingui Liu: City University of Hong Kong, Kowloon Tong
Nan Wu: Northwest University
Xiaolong Shao: City University of Hong Kong, Kowloon Tong
Yimeng Yin: Shanghai Fourth People’s Hospital Affiliated to Tongji University School of Medicine
Jian Yan: Northwest University
Xin Deng: City University of Hong Kong, Kowloon Tong

Nature Communications, 2020, vol. 11, issue 1, 1-11

Abstract: Abstract Pseudomonas syringae is a Gram-negative and model pathogenic bacterium that causes plant diseases worldwide. Here, we set out to identify binding motifs for all 301 annotated transcription factors (TFs) of P. syringae using HT-SELEX. We successfully identify binding motifs for 100 TFs. We map functional interactions between the TFs and their targets in virulence-associated pathways, and validate many of these interactions and functions using additional methods such as ChIP-seq, electrophoretic mobility shift assay (EMSA), RT-qPCR, and reporter assays. Our work identifies 25 virulence-associated master regulators, 14 of which had not been characterized as TFs before.

Date: 2020
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-020-18744-7 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-18744-7

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-020-18744-7

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().