Monitoring and modeling of lymphocytic leukemia cell bioenergetics reveals decreased ATP synthesis during cell division

Joon Ho Kang, Georgios Katsikis, Zhaoqi Li, Kiera M. Sapp, Max A. Stockslager, Daniel Lim, Matthew G. Heiden, Michael B. Yaffe, Scott R. Manalis and Teemu P. Miettinen ()
Additional contact information
Joon Ho Kang: Massachusetts Institute of Technology
Georgios Katsikis: Massachusetts Institute of Technology
Zhaoqi Li: Massachusetts Institute of Technology
Kiera M. Sapp: Massachusetts Institute of Technology
Max A. Stockslager: Massachusetts Institute of Technology
Daniel Lim: Massachusetts Institute of Technology
Matthew G. Heiden: Massachusetts Institute of Technology
Michael B. Yaffe: Massachusetts Institute of Technology
Scott R. Manalis: Massachusetts Institute of Technology
Teemu P. Miettinen: Massachusetts Institute of Technology

Nature Communications, 2020, vol. 11, issue 1, 1-13

Abstract: Abstract The energetic demands of a cell are believed to increase during mitosis, but the rates of ATP synthesis and consumption during mitosis have not been quantified. Here, we monitor mitochondrial membrane potential of single lymphocytic leukemia cells and demonstrate that mitochondria hyperpolarize from the G2/M transition until the metaphase-anaphase transition. This hyperpolarization was dependent on cyclin-dependent kinase 1 (CDK1) activity. By using an electrical circuit model of mitochondria, we quantify mitochondrial ATP synthesis rates in mitosis from the single-cell time-dynamics of mitochondrial membrane potential. We find that mitochondrial ATP synthesis decreases by approximately 50% during early mitosis and increases back to G2 levels during cytokinesis. Consistently, ATP levels and ATP synthesis are lower in mitosis than in G2 in synchronized cell populations. Overall, our results provide insights into mitotic bioenergetics and suggest that cell division is not a highly energy demanding process.

Date: 2020
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-020-18769-y Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-18769-y

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-020-18769-y

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().