Immunological fingerprint of 4CMenB recombinant antigens via protein microarray reveals key immunosignatures correlating with bactericidal activity

E. Bartolini (), E. Borgogni, M. Bruttini, A. Muzzi, M. Giuliani, S. Iozzi, R. Petracca, M. Martinelli, S. Bonacci, S. Marchi, C. Brettoni, C. Donati, G. Torricelli, S. Guidotti, M. Domina, C. Beninati, G. Teti, F. Felici, R. Rappuoli, F. Castellino, G. Giudice, V. Masignani, M. Pizza and D. Maione ()
Additional contact information
E. Bartolini: GSK
E. Borgogni: GSK
M. Bruttini: GSK
A. Muzzi: GSK
M. Giuliani: GSK
S. Iozzi: GSK
R. Petracca: GSK
M. Martinelli: GSK
S. Bonacci: GSK
S. Marchi: GSK
C. Brettoni: GSK
C. Donati: GSK
G. Torricelli: GSK
S. Guidotti: GSK
M. Domina: University of Messina
C. Beninati: University of Messina
G. Teti: University of Messina
F. Felici: University of Molise
R. Rappuoli: GSK
F. Castellino: GSK
G. Giudice: GSK
V. Masignani: GSK
M. Pizza: GSK
D. Maione: GSK

Nature Communications, 2020, vol. 11, issue 1, 1-12

Abstract: Abstract Serogroup B meningococcus (MenB) is a leading cause of meningitis and sepsis across the world and vaccination is the most effective way to protect against this disease. 4CMenB is a multi-component vaccine against MenB, which is now licensed for use in subjects >2 months of age in several countries. In this study, we describe the development and use of an ad hoc protein microarray to study the immune response induced by the three major 4CMenB antigenic components (fHbp, NHBA and NadA) in individual sera from vaccinated infants, adolescents and adults. The resulting 4CMenB protein antigen fingerprinting allowed the identification of specific human antibody repertoire correlating with the bactericidal response elicited in each subject. This work represents an example of epitope mapping of the immune response induced by a multicomponent vaccine in different age groups with the identification of protective signatures. It shows the high flexibility of this microarray based methodology in terms of high-throughput information and minimal volume of biological samples needed.

Date: 2020
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-020-18791-0 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-18791-0

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-020-18791-0

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().