One-pot biocatalytic route from cycloalkanes to α,ω‐dicarboxylic acids by designed Escherichia coli consortia

Fei Wang, Jing Zhao, Qian Li, Jun Yang, Renjie Li, Jian Min, Xiaojuan Yu, Gao-Wei Zheng, Hui-Lei Yu, Chao Zhai, Carlos G. Acevedo-Rocha, Lixin Ma and Aitao Li ()
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Fei Wang: Hubei University
Jing Zhao: Hubei University
Qian Li: Hubei University
Jun Yang: Hubei University
Renjie Li: Hubei University
Jian Min: Hubei University
Xiaojuan Yu: Hubei University
Gao-Wei Zheng: East China University of Science and Technology
Hui-Lei Yu: East China University of Science and Technology
Chao Zhai: Hubei University
Carlos G. Acevedo-Rocha: Biosyntia ApS
Lixin Ma: Hubei University
Aitao Li: Hubei University

Nature Communications, 2020, vol. 11, issue 1, 1-10

Abstract: Abstract Aliphatic α,ω‐dicarboxylic acids (DCAs) are a class of useful chemicals that are currently produced by energy-intensive, multistage chemical oxidations that are hazardous to the environment. Therefore, the development of environmentally friendly, safe, neutral routes to DCAs is important. We report an in vivo artificially designed biocatalytic cascade process for biotransformation of cycloalkanes to DCAs. To reduce protein expression burden and redox constraints caused by multi-enzyme expression in a single microbe, the biocatalytic pathway is divided into three basic Escherichia coli cell modules. The modules possess either redox-neutral or redox-regeneration systems and are combined to form E. coli consortia for use in biotransformations. The designed consortia of E. coli containing the modules efficiently convert cycloalkanes or cycloalkanols to DCAs without addition of exogenous coenzymes. Thus, this developed biocatalytic process provides a promising alternative to the current industrial process for manufacturing DCAs.

Date: 2020
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DOI: 10.1038/s41467-020-18833-7

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