p50 mono-ubiquitination and interaction with BARD1 regulates cell cycle progression and maintains genome stability

Longtao Wu, Clayton D. Crawley, Andrea Garofalo, Jackie W. Nichols, Paige-Ashley Campbell, Galina F. Khramtsova, Olufunmilayo I. Olopade, Ralph R. Weichselbaum and Bakhtiar Yamini ()
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Longtao Wu: The University of Chicago
Clayton D. Crawley: The University of Chicago
Andrea Garofalo: The University of Chicago
Jackie W. Nichols: The University of Chicago
Paige-Ashley Campbell: The University of Chicago
Galina F. Khramtsova: The University of Chicago
Olufunmilayo I. Olopade: The University of Chicago
Ralph R. Weichselbaum: The University of Chicago
Bakhtiar Yamini: The University of Chicago

Nature Communications, 2020, vol. 11, issue 1, 1-16

Abstract: Abstract p50, the mature product of NFKB1, is constitutively produced from its precursor, p105. Here, we identify BARD1 as a p50-interacting factor. p50 directly associates with the BARD1 BRCT domains via a C-terminal phospho-serine motif. This interaction is induced by ATR and results in mono-ubiquitination of p50 by the BARD1/BRCA1 complex. During the cell cycle, p50 is mono-ubiquitinated in S phase and loss of this post-translational modification increases S phase progression and chromosomal breakage. Genome-wide studies reveal a substantial decrease in p50 chromatin enrichment in S phase and Cycln E is identified as a factor regulated by p50 during the G1 to S transition. Functionally, interaction with BARD1 promotes p50 protein stability and consistent with this, in human cancer specimens, low nuclear BARD1 protein strongly correlates with low nuclear p50. These data indicate that p50 mono-ubiquitination by BARD1/BRCA1 during the cell cycle regulates S phase progression to maintain genome integrity.

Date: 2020
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DOI: 10.1038/s41467-020-18838-2

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