CRISPRoff enables spatio-temporal control of CRISPR editing

Jared Carlson-Stevermer (), Reed Kelso, Anastasia Kadina, Sahil Joshi, Nicholas Rossi, John Walker, Rich Stoner and Travis Maures
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Jared Carlson-Stevermer: Synthego Corporation
Reed Kelso: Synthego Corporation
Anastasia Kadina: Synthego Corporation
Sahil Joshi: Synthego Corporation
Nicholas Rossi: Synthego Corporation
John Walker: Synthego Corporation
Rich Stoner: Synthego Corporation
Travis Maures: Synthego Corporation

Nature Communications, 2020, vol. 11, issue 1, 1-7

Abstract: Abstract Following introduction of CRISPR-Cas9 components into a cell, genome editing occurs unabated until degradation of its component nucleic acids and proteins by cellular processes. This uncontrolled reaction can lead to unintended consequences including off-target editing and chromosomal translocations. To address this, we develop a method for light-induced degradation of sgRNA termed CRISPRoff. Here we show that light-induced inactivation of ribonucleoprotein attenuates genome editing within cells and allows for titratable levels of editing efficiency and spatial patterning via selective illumination.

Date: 2020
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DOI: 10.1038/s41467-020-18853-3

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