A fast impedance-based antimicrobial susceptibility test

Daniel C. Spencer, Teagan F. Paton, Kieran T. Mulroney, Timothy J. J. Inglis, J. Mark Sutton and Hywel Morgan ()
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Daniel C. Spencer: University of Southampton
Teagan F. Paton: PathWest Laboratory Medicine
Kieran T. Mulroney: University of Western Australia
Timothy J. J. Inglis: PathWest Laboratory Medicine
J. Mark Sutton: National Infection Service
Hywel Morgan: University of Southampton

Nature Communications, 2020, vol. 11, issue 1, 1-11

Abstract: Abstract There is an urgent need to develop simple and fast antimicrobial susceptibility tests (ASTs) that allow informed prescribing of antibiotics. Here, we describe a label-free AST that can deliver results within an hour, using an actively dividing culture as starting material. The bacteria are incubated in the presence of an antibiotic for 30 min, and then approximately 105 cells are analysed one-by-one with microfluidic impedance cytometry for 2–3 min. The measured electrical characteristics reflect the phenotypic response of the bacteria to the mode of action of a particular antibiotic, in a 30-minute incubation window. The results are consistent with those obtained by classical broth microdilution assays for a range of antibiotics and bacterial species.

Date: 2020
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DOI: 10.1038/s41467-020-18902-x

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