Complex multicomponent patterns rendered on a 3D DNA-barrel pegboard

Shelley F. J. Wickham, Alexander Auer, Jianghong Min, Nandhini Ponnuswamy, Johannes B. Woehrstein, Florian Schueder, Maximilian T. Strauss, Jörg Schnitzbauer, Bhavik Nathwani, Zhao Zhao, Steven D. Perrault, Jaeseung Hahn, Seungwoo Lee, Maartje M. Bastings, Sarah W. Helmig, Anne Louise Kodal, Peng Yin, Ralf Jungmann () and William M. Shih ()
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Shelley F. J. Wickham: Harvard Medical School
Alexander Auer: Ludwig Maximilian University
Jianghong Min: Harvard Medical School
Nandhini Ponnuswamy: Harvard Medical School
Johannes B. Woehrstein: Ludwig Maximilian University
Florian Schueder: Ludwig Maximilian University
Maximilian T. Strauss: Ludwig Maximilian University
Jörg Schnitzbauer: Ludwig Maximilian University
Bhavik Nathwani: Harvard Medical School
Zhao Zhao: Harvard Medical School
Steven D. Perrault: Harvard Medical School
Jaeseung Hahn: Harvard Medical School
Seungwoo Lee: Harvard Medical School
Maartje M. Bastings: Harvard Medical School
Sarah W. Helmig: Aarhus University
Anne Louise Kodal: Aarhus University
Peng Yin: Wyss Institute for Biologically Inspired Engineering
Ralf Jungmann: Ludwig Maximilian University
William M. Shih: Harvard Medical School

Nature Communications, 2020, vol. 11, issue 1, 1-10

Abstract: Abstract DNA origami, in which a long scaffold strand is assembled with a many short staple strands into parallel arrays of double helices, has proven a powerful method for custom nanofabrication. However, currently the design and optimization of custom 3D DNA-origami shapes is a barrier to rapid application to new areas. Here we introduce a modular barrel architecture, and demonstrate hierarchical assembly of a 100 megadalton DNA-origami barrel of ~90 nm diameter and ~250 nm height, that provides a rhombic-lattice canvas of a thousand pixels each, with pitch of ~8 nm, on its inner and outer surfaces. Complex patterns rendered on these surfaces were resolved using up to twelve rounds of Exchange-PAINT super-resolution microscopy. We envision these structures as versatile nanoscale pegboards for applications requiring complex 3D arrangements of matter, which will serve to promote rapid uptake of this technology in diverse fields beyond specialist groups working in DNA nanotechnology.

Date: 2020
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DOI: 10.1038/s41467-020-18910-x

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