mTORC1 activation in lung mesenchyme drives sex- and age-dependent pulmonary structure and function decline

Kseniya Obraztsova, Maria C. Basil, Ryan Rue, Aravind Sivakumar, Susan M. Lin, Alexander R. Mukhitov, Andrei I. Gritsiuta, Jilly F. Evans, Meghan Kopp, Jeremy Katzen, Annette Robichaud, Elena N. Atochina-Vasserman, Shanru Li, Justine Carl, Apoorva Babu, Michael P. Morley, Edward Cantu, Michael F. Beers, David B. Frank, Edward E. Morrisey and Vera P. Krymskaya ()
Additional contact information
Kseniya Obraztsova: University of Pennsylvania
Maria C. Basil: University of Pennsylvania
Ryan Rue: University of Pennsylvania
Aravind Sivakumar: Children Hospital of Philadelphia
Susan M. Lin: University of Pennsylvania
Alexander R. Mukhitov: University of Pennsylvania
Andrei I. Gritsiuta: University of Pennsylvania
Jilly F. Evans: University of Pennsylvania
Meghan Kopp: University of Pennsylvania
Jeremy Katzen: University of Pennsylvania
Annette Robichaud: SCIREQ Inc.
Elena N. Atochina-Vasserman: University of Pennsylvania
Shanru Li: University of Pennsylvania
Justine Carl: University of Pennsylvania
Apoorva Babu: University of Pennsylvania
Michael P. Morley: University of Pennsylvania
Edward Cantu: University of Pennsylvania
Michael F. Beers: University of Pennsylvania
David B. Frank: University of Pennsylvania
Edward E. Morrisey: University of Pennsylvania
Vera P. Krymskaya: University of Pennsylvania

Nature Communications, 2020, vol. 11, issue 1, 1-15

Abstract: Abstract Lymphangioleiomyomatosis (LAM) is a rare fatal cystic lung disease due to bi-allelic inactivating mutations in tuberous sclerosis complex (TSC1/TSC2) genes coding for suppressors of the mechanistic target of rapamycin complex 1 (mTORC1). The origin of LAM cells is still unknown. Here, we profile a LAM lung compared to an age- and sex-matched healthy control lung as a hypothesis-generating approach to identify cell subtypes that are specific to LAM. Our single-cell RNA sequencing (scRNA-seq) analysis reveals novel mesenchymal and transitional alveolar epithelial states unique to LAM lung. This analysis identifies a mesenchymal cell hub coordinating the LAM disease phenotype. Mesenchymal-restricted deletion of Tsc2 in the mouse lung produces a mTORC1-driven pulmonary phenotype, with a progressive disruption of alveolar structure, a decline in pulmonary function, increase of rapamycin-sensitive expression of WNT ligands, and profound female-specific changes in mesenchymal and epithelial lung cell gene expression. Genetic inactivation of WNT signaling reverses age-dependent changes of mTORC1-driven lung phenotype, but WNT activation alone in lung mesenchyme is not sufficient for the development of mouse LAM-like phenotype. The alterations in gene expression are driven by distinctive crosstalk between mesenchymal and epithelial subsets of cells observed in mesenchymal Tsc2-deficient lungs. This study identifies sex- and age-specific gene changes in the mTORC1-activated lung mesenchyme and establishes the importance of the WNT signaling pathway in the mTORC1-driven lung phenotype.

Date: 2020
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DOI: 10.1038/s41467-020-18979-4

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