Identification of key sequence features required for microRNA biogenesis in plants

Arantxa M. L. Rojas, Salvador I. Drusin, Uciel Chorostecki, Julieta L. Mateos, Belén Moro, Nicolas G. Bologna, Edgardo G. Bresso, Arnaldo Schapire, Rodolfo M. Rasia, Diego M. Moreno and Javier F. Palatnik ()
Additional contact information
Arantxa M. L. Rojas: IBR (Instituto de Biología Molecular y Celular de Rosario), CONICET and Universidad Nacional de Rosario
Salvador I. Drusin: IBR (Instituto de Biología Molecular y Celular de Rosario), CONICET and Universidad Nacional de Rosario
Uciel Chorostecki: IBR (Instituto de Biología Molecular y Celular de Rosario), CONICET and Universidad Nacional de Rosario
Julieta L. Mateos: IBR (Instituto de Biología Molecular y Celular de Rosario), CONICET and Universidad Nacional de Rosario
Belén Moro: IBR (Instituto de Biología Molecular y Celular de Rosario), CONICET and Universidad Nacional de Rosario
Nicolas G. Bologna: IBR (Instituto de Biología Molecular y Celular de Rosario), CONICET and Universidad Nacional de Rosario
Edgardo G. Bresso: IBR (Instituto de Biología Molecular y Celular de Rosario), CONICET and Universidad Nacional de Rosario
Arnaldo Schapire: IBR (Instituto de Biología Molecular y Celular de Rosario), CONICET and Universidad Nacional de Rosario
Rodolfo M. Rasia: IBR (Instituto de Biología Molecular y Celular de Rosario), CONICET and Universidad Nacional de Rosario
Diego M. Moreno: Instituto de Química de Rosario (CONICET-UNR)
Javier F. Palatnik: IBR (Instituto de Biología Molecular y Celular de Rosario), CONICET and Universidad Nacional de Rosario

Nature Communications, 2020, vol. 11, issue 1, 1-11

Abstract: Abstract MicroRNAs (miRNAs) are endogenous small RNAs of ∼21 nt that regulate multiple biological pathways in multicellular organisms. They derive from longer transcripts that harbor an imperfect stem-loop structure. In plants, the ribonuclease type III DICER-LIKE1 assisted by accessory proteins cleaves the precursor to release the mature miRNA. Numerous studies highlight the role of the precursor secondary structure during plant miRNA biogenesis; however, little is known about the relevance of the precursor sequence. Here, we analyzed the sequence composition of plant miRNA primary transcripts and found specifically located sequence biases. We show that changes in the identity of specific nucleotides can increase or abolish miRNA biogenesis. Most conspicuously, our analysis revealed that the identity of the nucleotides at unpaired positions of the precursor plays a crucial role during miRNA biogenesis in Arabidopsis.

Date: 2020
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-020-19129-6 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-19129-6

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-020-19129-6

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().