Marcksl1 modulates endothelial cell mechanoresponse to haemodynamic forces to control blood vessel shape and size

Igor Kondrychyn, Douglas J. Kelly, Núria Taberner Carretero, Akane Nomori, Kagayaki Kato, Jeronica Chong, Hiroyuki Nakajima, Satoru Okuda, Naoki Mochizuki and Li-Kun Phng ()
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Igor Kondrychyn: RIKEN Center for Biosystems Dynamics Research
Douglas J. Kelly: RIKEN Center for Biosystems Dynamics Research
Núria Taberner Carretero: RIKEN Center for Biosystems Dynamics Research
Akane Nomori: RIKEN Center for Biosystems Dynamics Research
Kagayaki Kato: National Institutes of Natural Sciences
Jeronica Chong: RIKEN Center for Biosystems Dynamics Research
Hiroyuki Nakajima: National Cerebral and Cardiovascular Center, Research Institute
Satoru Okuda: WPI Nano Life Science Institute, Kanazawa University
Naoki Mochizuki: National Cerebral and Cardiovascular Center, Research Institute
Li-Kun Phng: RIKEN Center for Biosystems Dynamics Research

Nature Communications, 2020, vol. 11, issue 1, 1-18

Abstract: Abstract The formation of vascular tubes is driven by extensive changes in endothelial cell (EC) shape. Here, we have identified a role of the actin-binding protein, Marcksl1, in modulating the mechanical properties of EC cortex to regulate cell shape and vessel structure during angiogenesis. Increasing and depleting Marcksl1 expression level in vivo results in an increase and decrease, respectively, in EC size and the diameter of microvessels. Furthermore, endothelial overexpression of Marcksl1 induces ectopic blebbing on both apical and basal membranes, during and after lumen formation, that is suppressed by reduced blood flow. High resolution imaging reveals that Marcksl1 promotes the formation of linear actin bundles and decreases actin density at the EC cortex. Our findings demonstrate that a balanced network of linear and branched actin at the EC cortex is essential in conferring cortical integrity to resist the deforming forces of blood flow to regulate vessel structure.

Date: 2020
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-19308-5

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DOI: 10.1038/s41467-020-19308-5

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