A single-cell atlas and lineage analysis of the adult Drosophila ovary

Katja Rust, Lauren E. Byrnes, Kevin Shengyang Yu, Jason S. Park, Julie B. Sneddon, Aaron D. Tward and Todd G. Nystul ()
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Katja Rust: UCSF, Department of Anatomy
Lauren E. Byrnes: UCSF, Department of Anatomy
Kevin Shengyang Yu: UCSF, Department of Otolaryngology-Head and Neck Surgery
Jason S. Park: UCSF, Department of Otolaryngology-Head and Neck Surgery
Julie B. Sneddon: UCSF, Department of Anatomy
Aaron D. Tward: UCSF, Department of Otolaryngology-Head and Neck Surgery
Todd G. Nystul: UCSF, Department of Anatomy

Nature Communications, 2020, vol. 11, issue 1, 1-17

Abstract: Abstract The Drosophila ovary is a widely used model for germ cell and somatic tissue biology. Here we use single-cell RNA-sequencing (scRNA-seq) to build a comprehensive cell atlas of the adult Drosophila ovary that contains transcriptional profiles for every major cell type in the ovary, including the germline stem cells and their niche cells, follicle stem cells, and previously undescribed subpopulations of escort cells. In addition, we identify Gal4 lines with specific expression patterns and perform lineage tracing of subpopulations of escort cells and follicle cells. We discover that a distinct subpopulation of escort cells is able to convert to follicle stem cells in response to starvation or upon genetic manipulation, including knockdown of escargot, or overactivation of mTor or Toll signalling.

Date: 2020
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-19361-0

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DOI: 10.1038/s41467-020-19361-0

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