Developing a pathway-independent and full-autonomous global resource allocation strategy to dynamically switching phenotypic states

Junjun Wu (), Meijiao Bao, Xuguo Duan, Peng Zhou, Caiwen Chen, Jiahua Gao, Shiyao Cheng, Qianqian Zhuang and Zhijun Zhao
Additional contact information
Junjun Wu: Nanjing Agricultural University
Meijiao Bao: Nanjing Agricultural University
Xuguo Duan: Nanjing Forestry University
Peng Zhou: Nanjing Agricultural University
Caiwen Chen: Nanjing Agricultural University
Jiahua Gao: Nanjing Agricultural University
Shiyao Cheng: Nanjing Agricultural University
Qianqian Zhuang: School of Bioengineering, Qilu University of Technology
Zhijun Zhao: Biorefinery Laboratory, Shanghai Advanced Research Institute, Chinese Academy of Sciences

Nature Communications, 2020, vol. 11, issue 1, 1-14

Abstract: Abstract A grand challenge of biological chemical production is the competition between synthetic circuits and host genes for limited cellular resources. Quorum sensing (QS)-based dynamic pathway regulations provide a pathway-independent way to rebalance metabolic flux over the course of the fermentation. Most cases, however, these pathway-independent strategies only have capacity for a single QS circuit functional in one cell. Furthermore, current dynamic regulations mainly provide localized control of metabolic flux. Here, with the aid of engineering synthetic orthogonal quorum-related circuits and global mRNA decay, we report a pathway-independent dynamic resource allocation strategy, which allows us to independently controlling two different phenotypic states to globally redistribute cellular resources toward synthetic circuits. The strategy which could pathway-independently and globally self-regulate two desired cell phenotypes including growth and production phenotypes could totally eliminate the need for human supervision of the entire fermentation.

Date: 2020
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-020-19432-2 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-19432-2

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-020-19432-2

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().