The smfBox is an open-source platform for single-molecule FRET

Benjamin Ambrose, James M. Baxter, John Cully, Matthew Willmott, Elliot M. Steele, Benji C. Bateman, Marisa L. Martin-Fernandez, Ashley Cadby, Jonathan Shewring, Marleen Aaldering and Timothy D. Craggs ()
Additional contact information
Benjamin Ambrose: University of Sheffield
James M. Baxter: University of Sheffield
John Cully: University of Sheffield
Matthew Willmott: University of Sheffield
Elliot M. Steele: University of Sheffield
Benji C. Bateman: Central Laser Facility, Research Complex at Harwell, Rutherford Appleton Laboratory
Marisa L. Martin-Fernandez: Central Laser Facility, Research Complex at Harwell, Rutherford Appleton Laboratory
Ashley Cadby: University of Sheffield
Jonathan Shewring: University of Sheffield
Marleen Aaldering: University of Sheffield
Timothy D. Craggs: University of Sheffield

Nature Communications, 2020, vol. 11, issue 1, 1-6

Abstract: Abstract Single-molecule Förster Resonance Energy Transfer (smFRET) is a powerful technique capable of resolving both relative and absolute distances within and between structurally dynamic biomolecules. High instrument costs, and a lack of open-source hardware and acquisition software have limited smFRET’s broad application by non-specialists. Here, we present the smfBox, a cost-effective confocal smFRET platform, providing detailed build instructions, open-source acquisition software, and full validation, thereby democratising smFRET for the wider scientific community.

Date: 2020
References: Add references at CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
https://www.nature.com/articles/s41467-020-19468-4 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-19468-4

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-020-19468-4

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().