Organized cannabinoid receptor distribution in neurons revealed by super-resolution fluorescence imaging

Hui Li, Jie Yang, Cuiping Tian, Min Diao, Quan Wang, Simeng Zhao, Shanshan Li, Fangzhi Tan, Tian Hua, Ya Qin, Chao-Po Lin, Dylan Deska-Gauthier, Garth J. Thompson, Ying Zhang, Wenqing Shui, Zhi-Jie Liu, Tong Wang and Guisheng Zhong ()
Additional contact information
Hui Li: ShanghaiTech University
Jie Yang: ShanghaiTech University
Cuiping Tian: ShanghaiTech University
Min Diao: ShanghaiTech University
Quan Wang: ShanghaiTech University
Simeng Zhao: ShanghaiTech University
Shanshan Li: ShanghaiTech University
Fangzhi Tan: ShanghaiTech University
Tian Hua: ShanghaiTech University
Ya Qin: ShanghaiTech University
Chao-Po Lin: ShanghaiTech University
Dylan Deska-Gauthier: Dalhousie University
Garth J. Thompson: ShanghaiTech University
Ying Zhang: Dalhousie University
Wenqing Shui: ShanghaiTech University
Zhi-Jie Liu: ShanghaiTech University
Tong Wang: ShanghaiTech University
Guisheng Zhong: ShanghaiTech University

Nature Communications, 2020, vol. 11, issue 1, 1-12

Abstract: Abstract G-protein-coupled receptors (GPCRs) play important roles in cellular functions. However, their intracellular organization is largely unknown. Through investigation of the cannabinoid receptor 1 (CB1), we discovered periodically repeating clusters of CB1 hotspots within the axons of neurons. We observed these CB1 hotspots interact with the membrane-associated periodic skeleton (MPS) forming a complex crucial in the regulation of CB1 signaling. Furthermore, we found that CB1 hotspot periodicity increased upon CB1 agonist application, and these activated CB1 displayed less dynamic movement compared to non-activated CB1. Our results suggest that CB1 forms periodic hotspots organized by the MPS as a mechanism to increase signaling efficacy upon activation.

Date: 2020
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DOI: 10.1038/s41467-020-19510-5

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