Subnuclear gene positioning through lamina association affects copper tolerance

Yuki Sakamoto, Mayuko Sato, Yoshikatsu Sato, Akihito Harada, Takamasa Suzuki, Chieko Goto, Kentaro Tamura, Kiminori Toyooka, Hiroshi Kimura, Yasuyuki Ohkawa, Ikuko Hara-Nishimura, Shingo Takagi and Sachihiro Matsunaga ()
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Yuki Sakamoto: Tokyo University of Science
Mayuko Sato: RIKEN Center for Sustainable Resource Science
Yoshikatsu Sato: Nagoya University
Akihito Harada: Kyushu University
Takamasa Suzuki: Chubu University
Chieko Goto: Graduate School of Agricultural and Life Sciences, The University of Tokyo
Kentaro Tamura: University of Shizuoka
Kiminori Toyooka: RIKEN Center for Sustainable Resource Science
Hiroshi Kimura: Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology
Yasuyuki Ohkawa: Kyushu University
Ikuko Hara-Nishimura: Konan University
Shingo Takagi: Osaka University
Sachihiro Matsunaga: Tokyo University of Science

Nature Communications, 2020, vol. 11, issue 1, 1-12

Abstract: Abstract The nuclear lamina plays an important role in the regulation of chromatin organization and gene positioning in animals. CROWDED NUCLEI (CRWN) is a strong candidate for the plant nuclear lamina protein in Arabidopsis thaliana but its biological function was largely unknown. Here, we show that CRWNs localize at the nuclear lamina and build the meshwork structure. Fluorescence in situ hybridization and RNA-seq analyses revealed that CRWNs regulate chromatin distribution and gene expression. More than 2000 differentially expressed genes were identified in the crwn1crwn4 double mutant. Copper-associated (CA) genes that form a gene cluster on chromosome 5 were among the downregulated genes in the double mutant exhibiting low tolerance to excess copper. Our analyses showed this low tolerance to copper was associated with the suppression of CA gene expression and that CRWN1 interacts with the CA gene locus, enabling the locus to localize at the nuclear lamina under excess copper conditions.

Date: 2020
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DOI: 10.1038/s41467-020-19621-z

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