Plant hairy roots enable high throughput identification of antimicrobials against Candidatus Liberibacter spp

Sonia Irigoyen, Manikandan Ramasamy, Shankar Pant, Prakash Niraula, Renesh Bedre, Meena Gurung, Denise Rossi, Corinne Laughlin, Zachary Gorman, Diann Achor, Amit Levy, Michael V. Kolomiets, Mamoudou Sétamou, Ismael E. Badillo-Vargas, Carlos A. Avila, Michael S. Irey and Kranthi K. Mandadi ()
Additional contact information
Sonia Irigoyen: Texas A&M AgriLife Research and Extension Center
Manikandan Ramasamy: Texas A&M AgriLife Research and Extension Center
Shankar Pant: Texas A&M AgriLife Research and Extension Center
Prakash Niraula: Texas A&M AgriLife Research and Extension Center
Renesh Bedre: Texas A&M AgriLife Research and Extension Center
Meena Gurung: Texas A&M AgriLife Research and Extension Center
Denise Rossi: Texas A&M AgriLife Research and Extension Center
Corinne Laughlin: Texas A&M AgriLife Research and Extension Center
Zachary Gorman: Texas A&M University
Diann Achor: University of Florida
Amit Levy: University of Florida
Michael V. Kolomiets: Texas A&M University
Mamoudou Sétamou: Texas A&M University-Kingsville, Citrus Center
Ismael E. Badillo-Vargas: Texas A&M AgriLife Research and Extension Center
Carlos A. Avila: Texas A&M AgriLife Research and Extension Center
Michael S. Irey: Southern Gardens Citrus
Kranthi K. Mandadi: Texas A&M AgriLife Research and Extension Center

Nature Communications, 2020, vol. 11, issue 1, 1-14

Abstract: Abstract A major bottleneck in identifying therapies to control citrus greening and other devastating plant diseases caused by fastidious pathogens is our inability to culture the pathogens in defined media or axenic cultures. As such, conventional approaches for antimicrobial evaluation (genetic or chemical) rely on time-consuming, low-throughput and inherently variable whole-plant assays. Here, we report that plant hairy roots support the growth of fastidious pathogens like Candidatus Liberibacter spp., the presumptive causal agents of citrus greening, potato zebra chip and tomato vein greening diseases. Importantly, we leverage the microbial hairy roots for rapid, reproducible efficacy screening of multiple therapies. We identify six antimicrobial peptides, two plant immune regulators and eight chemicals which inhibit Candidatus Liberibacter spp. in plant tissues. The antimicrobials, either singly or in combination, can be used as near- and long-term therapies to control citrus greening, potato zebra chip and tomato vein greening diseases.

Date: 2020
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DOI: 10.1038/s41467-020-19631-x

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