High-dimensional super-resolution imaging reveals heterogeneity and dynamics of subcellular lipid membranes

Karl Zhanghao (), Wenhui Liu, Meiqi Li, Zihan Wu, Xiao Wang, Xingye Chen, Chunyan Shan, Haoqian Wang, Xiaowei Chen, Qionghai Dai, Peng Xi () and Dayong Jin ()
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Karl Zhanghao: Southern University of Science and Technology
Wenhui Liu: Tsinghua University
Meiqi Li: Peking University
Zihan Wu: Southern University of Science and Technology
Xiao Wang: Peking University
Xingye Chen: Tsinghua University
Chunyan Shan: Peking University
Haoqian Wang: Tsinghua University
Xiaowei Chen: Peking University
Qionghai Dai: Tsinghua University
Peng Xi: Southern University of Science and Technology
Dayong Jin: Southern University of Science and Technology

Nature Communications, 2020, vol. 11, issue 1, 1-10

Abstract: Abstract Lipid membranes are found in most intracellular organelles, and their heterogeneities play an essential role in regulating the organelles’ biochemical functionalities. Here we report a Spectrum and Polarization Optical Tomography (SPOT) technique to study the subcellular lipidomics in live cells. Simply using one dye that universally stains the lipid membranes, SPOT can simultaneously resolve the membrane morphology, polarity, and phase from the three optical-dimensions of intensity, spectrum, and polarization, respectively. These high-throughput optical properties reveal lipid heterogeneities of ten subcellular compartments, at different developmental stages, and even within the same organelle. Furthermore, we obtain real-time monitoring of the multi-organelle interactive activities of cell division and successfully reveal their sophisticated lipid dynamics during the plasma membrane separation, tunneling nanotubules formation, and mitochondrial cristae dissociation. This work suggests research frontiers in correlating single-cell super-resolution lipidomics with multiplexed imaging of organelle interactome.

Date: 2020
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DOI: 10.1038/s41467-020-19747-0

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