Ultrasensitive antibody-aptamer plasmonic biosensor for malaria biomarker detection in whole blood

Antonio Minopoli, Bartolomeo Della Ventura, Bohdan Lenyk, Francesco Gentile, Julian A. Tanner, Andreas Offenhäusser, Dirk Mayer () and Raffaele Velotta ()
Additional contact information
Antonio Minopoli: Institute of Biological Information Processing (IBI-3), Bioelectronics, Forschungszentrum Jülich
Bartolomeo Della Ventura: University of Naples “Federico II”
Bohdan Lenyk: Institute of Biological Information Processing (IBI-3), Bioelectronics, Forschungszentrum Jülich
Francesco Gentile: University Magna Graecia
Julian A. Tanner: University of Hong Kong
Andreas Offenhäusser: Institute of Biological Information Processing (IBI-3), Bioelectronics, Forschungszentrum Jülich
Dirk Mayer: Institute of Biological Information Processing (IBI-3), Bioelectronics, Forschungszentrum Jülich
Raffaele Velotta: University of Naples “Federico II”

Nature Communications, 2020, vol. 11, issue 1, 1-10

Abstract: Abstract Development of plasmonic biosensors combining reliability and ease of use is still a challenge. Gold nanoparticle arrays made by block copolymer micelle nanolithography (BCMN) stand out for their scalability, cost-effectiveness and tunable plasmonic properties, making them ideal substrates for fluorescence enhancement. Here, we describe a plasmon-enhanced fluorescence immunosensor for the specific and ultrasensitive detection of Plasmodium falciparum lactate dehydrogenase (PfLDH)—a malaria marker—in whole blood. Analyte recognition is realized by oriented antibodies immobilized in a close-packed configuration via the photochemical immobilization technique (PIT), with a top bioreceptor of nucleic acid aptamers recognizing a different surface of PfLDH in a sandwich conformation. The combination of BCMN and PIT enabled maximum control over the nanoparticle size and lattice constant as well as the distance of the fluorophore from the sensing surface. The device achieved a limit of detection smaller than 1 pg/mL (

Date: 2020
References: Add references at CitEc
Citations: View citations in EconPapers (2)

Downloads: (external link)
https://www.nature.com/articles/s41467-020-19755-0 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-19755-0

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-020-19755-0

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().