In vivo biomolecular imaging of zebrafish embryos using confocal Raman spectroscopy

Håkon Høgset, Conor C. Horgan, James P. K. Armstrong, Mads S. Bergholt, Vincenzo Torraca, Qu Chen, Timothy J. Keane, Laurence Bugeon, Margaret J. Dallman, Serge Mostowy and Molly M. Stevens ()
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Håkon Høgset: Imperial College London
Conor C. Horgan: Imperial College London
James P. K. Armstrong: Imperial College London
Mads S. Bergholt: Imperial College London
Vincenzo Torraca: London School of Hygiene & Tropical Medicine
Qu Chen: Imperial College London
Timothy J. Keane: Imperial College London
Laurence Bugeon: Imperial College London
Margaret J. Dallman: Imperial College London
Serge Mostowy: London School of Hygiene & Tropical Medicine
Molly M. Stevens: Imperial College London

Nature Communications, 2020, vol. 11, issue 1, 1-12

Abstract: Abstract Zebrafish embryos provide a unique opportunity to visualize complex biological processes, yet conventional imaging modalities are unable to access intricate biomolecular information without compromising the integrity of the embryos. Here, we report the use of confocal Raman spectroscopic imaging for the visualization and multivariate analysis of biomolecular information extracted from unlabeled zebrafish embryos. We outline broad applications of this method in: (i) visualizing the biomolecular distribution of whole embryos in three dimensions, (ii) resolving anatomical features at subcellular spatial resolution, (iii) biomolecular profiling and discrimination of wild type and ΔRD1 mutant Mycobacterium marinum strains in a zebrafish embryo model of tuberculosis and (iv) in vivo temporal monitoring of the wound response in living zebrafish embryos. Overall, this study demonstrates the application of confocal Raman spectroscopic imaging for the comparative bimolecular analysis of fully intact and living zebrafish embryos.

Date: 2020
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DOI: 10.1038/s41467-020-19827-1

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