Cohesin depleted cells rebuild functional nuclear compartments after endomitosis

Marion Cremer (), Katharina Brandstetter, Andreas Maiser, Suhas S. P. Rao, Volker J. Schmid, Miguel Guirao-Ortiz, Namita Mitra, Stefania Mamberti, Kyle N. Klein, David M. Gilbert, Heinrich Leonhardt, M. Cristina Cardoso, Erez Lieberman Aiden, Hartmann Harz () and Thomas Cremer ()
Additional contact information
Marion Cremer: Ludwig-Maximilians-Universität München
Katharina Brandstetter: Ludwig-Maximilians-Universität München
Andreas Maiser: Ludwig-Maximilians-Universität München
Suhas S. P. Rao: Baylor College of Medicine
Volker J. Schmid: Ludwig-Maximilians-Universität München
Miguel Guirao-Ortiz: Ludwig-Maximilians-Universität München
Namita Mitra: Baylor College of Medicine
Stefania Mamberti: Technische Universität Darmstadt
Kyle N. Klein: Florida State University
David M. Gilbert: Florida State University
Heinrich Leonhardt: Ludwig-Maximilians-Universität München
M. Cristina Cardoso: Technische Universität Darmstadt
Erez Lieberman Aiden: Baylor College of Medicine
Hartmann Harz: Ludwig-Maximilians-Universität München
Thomas Cremer: Ludwig-Maximilians-Universität München

Nature Communications, 2020, vol. 11, issue 1, 1-16

Abstract: Abstract Cohesin plays an essential role in chromatin loop extrusion, but its impact on a compartmentalized nuclear architecture, linked to nuclear functions, is less well understood. Using live-cell and super-resolved 3D microscopy, here we find that cohesin depletion in a human colon cancer derived cell line results in endomitosis and a single multilobulated nucleus with chromosome territories pervaded by interchromatin channels. Chromosome territories contain chromatin domain clusters with a zonal organization of repressed chromatin domains in the interior and transcriptionally competent domains located at the periphery. These clusters form microscopically defined, active and inactive compartments, which likely correspond to A/B compartments, which are detected with ensemble Hi-C. Splicing speckles are observed nearby within the lining channel system. We further observe that the multilobulated nuclei, despite continuous absence of cohesin, pass through S-phase with typical spatio-temporal patterns of replication domains. Evidence for structural changes of these domains compared to controls suggests that cohesin is required for their full integrity.

Date: 2020
References: Add references at CitEc
Citations: View citations in EconPapers (3)

Downloads: (external link)
https://www.nature.com/articles/s41467-020-19876-6 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-19876-6

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-020-19876-6

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().