An expanded palette of improved SPLICS reporters detects multiple organelle contacts in vitro and in vivo

Francesca Vallese, Cristina Catoni, Domenico Cieri, Lucia Barazzuol, Omar Ramirez, Valentina Calore, Massimo Bonora, Flavia Giamogante, Paolo Pinton, Marisa Brini () and Tito Calì ()
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Francesca Vallese: University of Padova
Cristina Catoni: University of Padova
Domenico Cieri: University of Padova
Lucia Barazzuol: University of Padova
Omar Ramirez: Interdisciplinary Center for Neurosciences, Heidelberg University
Valentina Calore: University of Padova
Massimo Bonora: Surgery and Experimental Medicine, Section of General Pathology, University of Ferrara
Flavia Giamogante: University of Padova
Paolo Pinton: Surgery and Experimental Medicine, Section of General Pathology, University of Ferrara
Marisa Brini: University of Padova
Tito Calì: University of Padova

Nature Communications, 2020, vol. 11, issue 1, 1-15

Abstract: Abstract Membrane contact sites between virtually any known organelle have been documented and, in the last decades, their study received momentum due to their importance for fundamental activities of the cell and for the subtle comprehension of many human diseases. The lack of tools to finely image inter-organelle proximity hindered our understanding on how these subcellular communication hubs mediate and regulate cell homeostasis. We develop an improved and expanded palette of split-GFP-based contact site sensors (SPLICS) for the detection of single and multiple organelle contact sites within a scalable distance range. We demonstrate their flexibility under physiological conditions and in living organisms.

Date: 2020
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-19892-6

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DOI: 10.1038/s41467-020-19892-6

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