Disposable silicon-based all-in-one micro-qPCR for rapid on-site detection of pathogens

Estefania Nunez-Bajo, Alexander Silva Pinto Collins, Michael Kasimatis, Yasin Cotur, Tarek Asfour, Ugur Tanriverdi, Max Grell, Matti Kaisti, Guglielmo Senesi, Karen Stevenson and Firat Güder ()
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Estefania Nunez-Bajo: Imperial College London
Alexander Silva Pinto Collins: Imperial College London
Michael Kasimatis: Imperial College London
Yasin Cotur: Imperial College London
Tarek Asfour: Imperial College London
Ugur Tanriverdi: Imperial College London
Max Grell: Imperial College London
Matti Kaisti: Imperial College London
Guglielmo Senesi: Imperial College London
Karen Stevenson: Moredun Research Institute, Pentlands Science Park, Bush Loan
Firat Güder: Imperial College London

Nature Communications, 2020, vol. 11, issue 1, 1-10

Abstract: Abstract Rapid screening and low-cost diagnosis play a crucial role in choosing the correct course of intervention when dealing with highly infectious pathogens. This is especially important if the disease-causing agent has no effective treatment, such as the novel coronavirus SARS-CoV-2, and shows no or similar symptoms to other common infections. Here, we report a disposable silicon-based integrated Point-of-Need transducer (TriSilix) for real-time quantitative detection of pathogen-specific sequences of nucleic acids. TriSilix can be produced at wafer-scale in a standard laboratory (37 chips of 10 × 10 × 0.65 mm in size can be produced in 7 h, costing ~0.35 USD per device). We are able to quantitatively detect a 563 bp fragment of genomic DNA of Mycobacterium avium subspecies paratuberculosis through real-time PCR with a limit-of-detection of 20 fg, equivalent to a single bacterium, at the 35th cycle. Using TriSilix, we also detect the cDNA from SARS-CoV-2 (1 pg) with high specificity against SARS-CoV (2003).

Date: 2020
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DOI: 10.1038/s41467-020-19911-6

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