TBPL2/TFIIA complex establishes the maternal transcriptome through oocyte-specific promoter usage

Changwei Yu, Nevena Cvetesic, Vincent Hisler, Kapil Gupta, Tao Ye, Emese Gazdag, Luc Negroni, Petra Hajkova, Imre Berger, Boris Lenhard, Ferenc Müller, Stéphane D. Vincent () and László Tora ()
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Changwei Yu: Institut de Génétique et de Biologie Moléculaire et Cellulaire
Nevena Cvetesic: Imperial College London, South Kensington Campus
Vincent Hisler: Institut de Génétique et de Biologie Moléculaire et Cellulaire
Kapil Gupta: University of Bristol, Cantock’s Close
Tao Ye: Institut de Génétique et de Biologie Moléculaire et Cellulaire
Emese Gazdag: Institut de Génétique et de Biologie Moléculaire et Cellulaire
Luc Negroni: Institut de Génétique et de Biologie Moléculaire et Cellulaire
Petra Hajkova: Imperial College London, South Kensington Campus
Imre Berger: University of Bristol, Cantock’s Close
Boris Lenhard: Imperial College London, South Kensington Campus
Ferenc Müller: University of Birmingham
Stéphane D. Vincent: Institut de Génétique et de Biologie Moléculaire et Cellulaire
László Tora: Institut de Génétique et de Biologie Moléculaire et Cellulaire

Nature Communications, 2020, vol. 11, issue 1, 1-13

Abstract: Abstract During oocyte growth, transcription is required to create RNA and protein reserves to achieve maternal competence. During this period, the general transcription factor TATA binding protein (TBP) is replaced by its paralogue, TBPL2 (TBP2 or TRF3), which is essential for RNA polymerase II transcription. We show that in oocytes TBPL2 does not assemble into a canonical TFIID complex. Our transcript analyses demonstrate that TBPL2 mediates transcription of oocyte-expressed genes, including mRNA survey genes, as well as specific endogenous retroviral elements. Transcription start site (TSS) mapping indicates that TBPL2 has a strong preference for TATA-like motif in core promoters driving sharp TSS selection, in contrast with canonical TBP/TFIID-driven TATA-less promoters that have broader TSS architecture. Thus, we show a role for the TBPL2/TFIIA complex in the establishment of the oocyte transcriptome by using a specific TSS recognition code.

Date: 2020
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DOI: 10.1038/s41467-020-20239-4

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