PRC2 and EHMT1 regulate H3K27me2 and H3K27me3 establishment across the zygote genome

Tie-Gang Meng, Qian Zhou, Xue-Shan Ma, Xiao-Yu Liu, Qing-Ren Meng, Xian-Ju Huang, Hong-Lin Liu, Wen-Long Lei, Zheng-Hui Zhao, Ying-Chun Ouyang, Yi Hou, Heide Schatten, Xiang-Hong Ou (), Zhen-Bo Wang (), Shao-Rong Gao () and Qing-Yuan Sun ()
Additional contact information
Tie-Gang Meng: Chinese Academy of Sciences
Qian Zhou: Chinese Academy of Sciences
Xue-Shan Ma: The First Affiliated Hospital of Zhengzhou University, Zhengzhou
Xiao-Yu Liu: Tongji University
Qing-Ren Meng: Chinese Academy of Sciences
Xian-Ju Huang: The First Affiliated Hospital of Zhengzhou University, Zhengzhou
Hong-Lin Liu: Nanjing Agricultural University
Wen-Long Lei: Chinese Academy of Sciences
Zheng-Hui Zhao: Chinese Academy of Sciences
Ying-Chun Ouyang: Chinese Academy of Sciences
Yi Hou: Chinese Academy of Sciences
Heide Schatten: University of Missouri
Xiang-Hong Ou: Guangdong Second Provincial General Hospital
Zhen-Bo Wang: Chinese Academy of Sciences
Shao-Rong Gao: Tongji University
Qing-Yuan Sun: Chinese Academy of Sciences

Nature Communications, 2020, vol. 11, issue 1, 1-12

Abstract: Abstract The formation of zygote is the beginning of mammalian life, and dynamic epigenetic modifications are essential for mammalian normal development. H3K27 di-methylation (H3K27me2) and H3K27 tri-methylation (H3K27me3) are marks of facultative heterochromatin which maintains transcriptional repression established during early development in many eukaryotes. However, the mechanism underlying establishment and regulation of epigenetic asymmetry in the zygote remains obscure. Here we show that maternal EZH2 is required for the establishment of H3K27me3 in mouse zygotes. However, combined immunostaining with ULI-NChIP-seq (ultra-low-input micrococcal nuclease-based native ChIP-seq) shows that EZH1 could partially safeguard the role of EZH2 in the formation of H3K27me2. Meanwhile, we identify that EHMT1 is involved in the establishment of H3K27me2, and that H3K27me2 might be an essential prerequisite for the following de novo H3K27me3 modification on the male pronucleus. In this work, we clarify the establishment and regulatory mechanisms of H3K27me2 and H3K27me3 in mouse zygotes.

Date: 2020
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DOI: 10.1038/s41467-020-20242-9

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