RETRACTED ARTICLE:The Arabidopsis NOT4A E3 ligase promotes PGR3 expression and regulates chloroplast translation

Mark Bailey, Aiste Ivanauskaite, Julia Grimmer, Oluwatunmise Akintewe, Adrienne C. Payne, Rory Osborne, Anne-Marie Labandera, Ross D. Etherington, Marjaana Rantala, Sacha Baginsky, Paula Mulo and Daniel J. Gibbs ()
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Mark Bailey: University of Birmingham
Aiste Ivanauskaite: University of Turku
Julia Grimmer: Martin-Luther-University Halle-Wittenberg
Oluwatunmise Akintewe: University of Birmingham
Adrienne C. Payne: University of Birmingham
Rory Osborne: University of Birmingham
Anne-Marie Labandera: University of Birmingham
Ross D. Etherington: University of Birmingham
Marjaana Rantala: University of Turku
Sacha Baginsky: Martin-Luther-University Halle-Wittenberg
Paula Mulo: University of Turku
Daniel J. Gibbs: University of Birmingham

Nature Communications, 2021, vol. 12, issue 1, 1-15

Abstract: Abstract Chloroplast function requires the coordinated action of nuclear- and chloroplast-derived proteins, including several hundred nuclear-encoded pentatricopeptide repeat (PPR) proteins that regulate plastid mRNA metabolism. Despite their large number and importance, regulatory mechanisms controlling PPR expression are poorly understood. Here we show that the Arabidopsis NOT4A ubiquitin-ligase positively regulates the expression of PROTON GRADIENT REGULATION 3 (PGR3), a PPR protein required for translating several thylakoid-localised photosynthetic components and ribosome subunits within chloroplasts. Loss of NOT4A function leads to a strong depletion of cytochrome b6f and NAD(P)H dehydrogenase (NDH) complexes, as well as plastid 30 S ribosomes, which reduces mRNA translation and photosynthetic capacity, causing pale-yellow and slow-growth phenotypes. Quantitative transcriptome and proteome analysis of the not4a mutant reveal it lacks PGR3 expression, and that its molecular defects resemble those of a pgr3 mutant. Furthermore, we show that normal plastid function is restored to not4a through transgenic PGR3 expression. Our work identifies NOT4A as crucial for ensuring robust photosynthetic function during development and stress-response, through promoting PGR3 production and chloroplast translation.

Date: 2021
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DOI: 10.1038/s41467-020-20506-4

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