Regulatory protein HilD stimulates Salmonella Typhimurium invasiveness by promoting smooth swimming via the methyl-accepting chemotaxis protein McpC

Kendal G. Cooper, Audrey Chong, Laszlo Kari, Brendan Jeffrey, Tregei Starr, Craig Martens, Molly McClurg, Victoria R. Posada, Richard C. Laughlin, Canaan Whitfield-Cargile, L. Garry Adams, Laura K. Bryan, Sara V. Little, Mary Krath, Sara D. Lawhon and Olivia Steele-Mortimer ()
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Kendal G. Cooper: National Institutes of Allergy and Infectious Diseases, National Institutes of Health
Audrey Chong: National Institutes of Allergy and Infectious Diseases, National Institutes of Health
Laszlo Kari: National Institutes of Allergy and Infectious Diseases, National Institutes of Health
Brendan Jeffrey: National Institutes of Allergy and Infectious Diseases, National Institutes of Health
Tregei Starr: National Institutes of Allergy and Infectious Diseases, National Institutes of Health
Craig Martens: National Institutes of Allergy and Infectious Diseases, National Institutes of Health
Molly McClurg: Texas A&M University-Kingsville
Victoria R. Posada: Texas A&M University-Kingsville
Richard C. Laughlin: Texas A&M University-Kingsville
Canaan Whitfield-Cargile: Texas A&M College of Veterinary Medicine and Biomedical Sciences
L. Garry Adams: Texas A&M College of Veterinary Medicine and Biomedical Sciences
Laura K. Bryan: Texas A&M College of Veterinary Medicine and Biomedical Sciences
Sara V. Little: Texas A&M College of Veterinary Medicine and Biomedical Sciences
Mary Krath: Texas A&M College of Veterinary Medicine and Biomedical Sciences
Sara D. Lawhon: Texas A&M College of Veterinary Medicine and Biomedical Sciences
Olivia Steele-Mortimer: National Institutes of Allergy and Infectious Diseases, National Institutes of Health

Nature Communications, 2021, vol. 12, issue 1, 1-12

Abstract: Abstract In the enteric pathogen Salmonella enterica serovar Typhimurium, invasion and motility are coordinated by the master regulator HilD, which induces expression of the type III secretion system 1 (T3SS1) and motility genes. Methyl-accepting chemotaxis proteins (MCPs) detect specific ligands and control the direction of the flagellar motor, promoting tumbling and changes in direction (if a repellent is detected) or smooth swimming (in the presence of an attractant). Here, we show that HilD induces smooth swimming by upregulating an uncharacterized MCP (McpC), and this is important for invasion of epithelial cells. Remarkably, in vitro assays show that McpC can suppress tumbling and increase smooth swimming in the absence of exogenous ligands. Expression of mcpC is repressed by the universal regulator H-NS, which can be displaced by HilD. Our results highlight the importance of smooth swimming for Salmonella Typhimurium invasiveness and indicate that McpC can act via a ligand-independent mechanism when incorporated into the chemotactic receptor array.

Date: 2021
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DOI: 10.1038/s41467-020-20558-6

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