Characterization of a new Leishmania major strain for use in a controlled human infection model

Helen Ashwin, Jovana Sadlova, Barbora Vojtkova, Tomas Becvar, Patrick Lypaczewski, Eli Schwartz, Elizabeth Greensted, Katrien Bocxlaer, Marion Pasin, Kai S. Lipinski, Vivak Parkash, Greg Matlashewski, Alison M. Layton, Charles J. Lacey, Charles L. Jaffe (), Petr Volf () and Paul M. Kaye ()
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Helen Ashwin: University of York
Jovana Sadlova: Charles University
Barbora Vojtkova: Charles University
Tomas Becvar: Charles University
Patrick Lypaczewski: McGill University
Eli Schwartz: Tel Aviv University
Elizabeth Greensted: University of York
Katrien Bocxlaer: University of York
Marion Pasin: Vibalogics GmbH
Kai S. Lipinski: Vibalogics GmbH
Vivak Parkash: University of York
Greg Matlashewski: McGill University
Alison M. Layton: University of York
Charles J. Lacey: University of York
Charles L. Jaffe: The Hebrew University-Hadassah Medical School
Petr Volf: Charles University
Paul M. Kaye: University of York

Nature Communications, 2021, vol. 12, issue 1, 1-12

Abstract: Abstract Leishmaniasis is widely regarded as a vaccine-preventable disease, but the costs required to reach pivotal Phase 3 studies and uncertainty about which candidate vaccines should be progressed into human studies significantly limits progress in vaccine development for this neglected tropical disease. Controlled human infection models (CHIMs) provide a pathway for accelerating vaccine development and to more fully understand disease pathogenesis and correlates of protection. Here, we describe the isolation, characterization and GMP manufacture of a new clinical strain of Leishmania major. Two fresh strains of L. major from Israel were initially compared by genome sequencing, in vivo infectivity and drug sensitivity in mice, and development and transmission competence in sand flies, allowing one to be selected for GMP production. This study addresses a major roadblock in the development of vaccines for leishmaniasis, providing a key resource for CHIM studies of sand fly transmitted cutaneous leishmaniasis.

Date: 2021
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DOI: 10.1038/s41467-020-20569-3

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