H2AK121ub in Arabidopsis associates with a less accessible chromatin state at transcriptional regulation hotspots

Xiaochang Yin, Francisco J. Romero-Campero (), Pedro de Los Reyes, Peng Yan, Jing Yang, Guangmei Tian, XiaoZeng Yang, Xiaorong Mo, Shuangshuang Zhao, Myriam Calonje () and Yue Zhou ()
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Xiaochang Yin: Peking University
Francisco J. Romero-Campero: Institute of Plant Biochemistry and Photosynthesis (IBVF-CSIC)
Pedro de Los Reyes: Institute of Plant Biochemistry and Photosynthesis (IBVF-CSIC)
Peng Yan: Zhejiang University
Jing Yang: Beijing Academy of Agriculture and Forestry Sciences
Guangmei Tian: Peking University
XiaoZeng Yang: Beijing Academy of Agriculture and Forestry Sciences
Xiaorong Mo: Zhejiang University
Shuangshuang Zhao: Shandong Normal University
Myriam Calonje: Institute of Plant Biochemistry and Photosynthesis (IBVF-CSIC)
Yue Zhou: Peking University

Nature Communications, 2021, vol. 12, issue 1, 1-12

Abstract: Abstract Although it is well established that the Polycomb Group (PcG) complexes maintain gene repression through the incorporation of H2AK121ub and H3K27me3, little is known about the effect of these modifications on chromatin accessibility, which is fundamental to understand PcG function. Here, by integrating chromatin accessibility, histone marks and expression analyses in different Arabidopsis PcG mutants, we show that PcG function regulates chromatin accessibility. We find that H2AK121ub is associated with a less accessible but still permissive chromatin at transcriptional regulation hotspots. Accessibility is further reduced by EMF1 acting in collaboration with PRC2 activity. Consequently, H2AK121ub/H3K27me3 marks are linked to inaccessible although responsive chromatin. In contrast, only-H3K27me3-marked chromatin is less responsive, indicating that H2AK121ub-marked hotspots are required for transcriptional responses. Nevertheless, despite the loss of PcG activities leads to increased chromatin accessibility, this is not necessarily accompanied by transcriptional activation, indicating that accessible chromatin is not always predictive of gene expression.

Date: 2021
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DOI: 10.1038/s41467-020-20614-1

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