Repeat DNA-PAINT suppresses background and non-specific signals in optical nanoscopy

Alexander H. Clowsley, William T. Kaufhold, Tobias Lutz, Anna Meletiou, Lorenzo Di Michele and Christian Soeller ()
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Alexander H. Clowsley: University of Exeter
William T. Kaufhold: University of Cambridge
Tobias Lutz: University of Exeter
Anna Meletiou: University of Exeter
Lorenzo Di Michele: Imperial College London
Christian Soeller: University of Exeter

Nature Communications, 2021, vol. 12, issue 1, 1-10

Abstract: Abstract DNA-PAINT is a versatile optical super-resolution technique relying on the transient binding of fluorescent DNA ‘imagers’ to target epitopes. Its performance in biological samples is often constrained by strong background signals and non-specific binding events, both exacerbated by high imager concentrations. Here we describe Repeat DNA-PAINT, a method that enables a substantial reduction in imager concentration, thus suppressing spurious signals. Additionally, Repeat DNA-PAINT reduces photoinduced target-site loss and can accelerate sampling, all without affecting spatial resolution.

Date: 2021
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DOI: 10.1038/s41467-020-20686-z

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