Degradation of complex arabinoxylans by human colonic Bacteroidetes

Gabriel V. Pereira, Ahmed M. Abdel-Hamid, Soumajit Dutta, Corina N. D’Alessandro-Gabazza, Daniel Wefers, Jacob A. Farris, Shiv Bajaj, Zdzislaw Wawrzak, Haruyuki Atomi, Roderick I. Mackie, Esteban C. Gabazza, Diwakar Shukla, Nicole M. Koropatkin and Isaac Cann ()
Additional contact information
Gabriel V. Pereira: Carl R. Woese Institute for Genomic Biology (Microbiome Metabolic Engineering Theme), University of Illinois at Urbana-Champaign
Ahmed M. Abdel-Hamid: Carl R. Woese Institute for Genomic Biology (Microbiome Metabolic Engineering Theme), University of Illinois at Urbana-Champaign
Soumajit Dutta: University of Illinois at Urbana-Champaign
Corina N. D’Alessandro-Gabazza: Carl R. Woese Institute for Genomic Biology (Microbiome Metabolic Engineering Theme), University of Illinois at Urbana-Champaign
Daniel Wefers: Carl R. Woese Institute for Genomic Biology (Microbiome Metabolic Engineering Theme), University of Illinois at Urbana-Champaign
Jacob A. Farris: Carl R. Woese Institute for Genomic Biology (Microbiome Metabolic Engineering Theme), University of Illinois at Urbana-Champaign
Shiv Bajaj: Carl R. Woese Institute for Genomic Biology (Microbiome Metabolic Engineering Theme), University of Illinois at Urbana-Champaign
Zdzislaw Wawrzak: Argonne National Laboratory
Haruyuki Atomi: Graduate School of Engineering, Kyoto University, Katsura
Roderick I. Mackie: Carl R. Woese Institute for Genomic Biology (Microbiome Metabolic Engineering Theme), University of Illinois at Urbana-Champaign
Esteban C. Gabazza: Carl R. Woese Institute for Genomic Biology (Microbiome Metabolic Engineering Theme), University of Illinois at Urbana-Champaign
Diwakar Shukla: University of Illinois at Urbana-Champaign
Nicole M. Koropatkin: Carl R. Woese Institute for Genomic Biology (Microbiome Metabolic Engineering Theme), University of Illinois at Urbana-Champaign
Isaac Cann: Carl R. Woese Institute for Genomic Biology (Microbiome Metabolic Engineering Theme), University of Illinois at Urbana-Champaign

Nature Communications, 2021, vol. 12, issue 1, 1-21

Abstract: Abstract Some Bacteroidetes and other human colonic bacteria can degrade arabinoxylans, common polysaccharides found in dietary fiber. Previous work has identified gene clusters (polysaccharide-utilization loci, PULs) for degradation of simple arabinoxylans. However, the degradation of complex arabinoxylans (containing side chains such as ferulic acid, a phenolic compound) is poorly understood. Here, we identify a PUL that encodes multiple esterases for degradation of complex arabinoxylans in Bacteroides species. The PUL is specifically upregulated in the presence of complex arabinoxylans. We characterize some of the esterases biochemically and structurally, and show that they release ferulic acid from complex arabinoxylans. Growth of four different colonic Bacteroidetes members, including Bacteroides intestinalis, on complex arabinoxylans results in accumulation of ferulic acid, a compound known to have antioxidative and immunomodulatory properties.

Date: 2021
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-020-20737-5

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DOI: 10.1038/s41467-020-20737-5

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