Structures of monomeric and dimeric PRC2:EZH1 reveal flexible modules involved in chromatin compaction

Daniel Grau, Yixiao Zhang, Chul-Hwan Lee, Marco Valencia-Sánchez, Jenny Zhang, Miao Wang, Marlene Holder, Vladimir Svetlov, Dongyan Tan, Evgeny Nudler, Danny Reinberg, Thomas Walz () and Karim-Jean Armache ()
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Daniel Grau: New York University Grossman School of Medicine
Yixiao Zhang: The Rockefeller University
Chul-Hwan Lee: New York University Grossman School of Medicine
Marco Valencia-Sánchez: New York University Grossman School of Medicine
Jenny Zhang: New York University Grossman School of Medicine
Miao Wang: New York University Grossman School of Medicine
Marlene Holder: New York University Grossman School of Medicine
Vladimir Svetlov: New York University Grossman School of Medicine
Dongyan Tan: Stony Brook University Medical School
Evgeny Nudler: New York University Grossman School of Medicine
Danny Reinberg: New York University Grossman School of Medicine
Thomas Walz: The Rockefeller University
Karim-Jean Armache: New York University Grossman School of Medicine

Nature Communications, 2021, vol. 12, issue 1, 1-12

Abstract: Abstract Polycomb repressive complex 2 (PRC2) is a histone methyltransferase critical for maintaining gene silencing during eukaryotic development. In mammals, PRC2 activity is regulated in part by the selective incorporation of one of two paralogs of the catalytic subunit, EZH1 or EZH2. Each of these enzymes has specialized biological functions that may be partially explained by differences in the multivalent interactions they mediate with chromatin. Here, we present two cryo-EM structures of PRC2:EZH1, one as a monomer and a second one as a dimer bound to a nucleosome. When bound to nucleosome substrate, the PRC2:EZH1 dimer undergoes a dramatic conformational change. We demonstrate that mutation of a divergent EZH1/2 loop abrogates the nucleosome-binding and methyltransferase activities of PRC2:EZH1. Finally, we show that PRC2:EZH1 dimers are more effective than monomers at promoting chromatin compaction, and the divergent EZH1/2 loop is essential for this function, thereby tying together the methyltransferase, nucleosome-binding, and chromatin-compaction activities of PRC2:EZH1. We speculate that the conformational flexibility and the ability to dimerize enable PRC2 to act on the varied chromatin substrates it encounters in the cell.

Date: 2021
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DOI: 10.1038/s41467-020-20775-z

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