A membrane-bound ankyrin repeat protein confers race-specific leaf rust disease resistance in wheat

Markus C. Kolodziej, Jyoti Singla, Javier Sánchez-Martín, Helen Zbinden, Hana Šimková, Miroslava Karafiátová, Jaroslav Doležel, Julien Gronnier, Manuel Poretti, Gaétan Glauser, Wangsheng Zhu, Philipp Köster, Cyril Zipfel, Thomas Wicker (), Simon G. Krattinger () and Beat Keller ()
Additional contact information
Markus C. Kolodziej: University of Zurich, Department of Plant and Microbial Biology
Jyoti Singla: University of Zurich, Department of Plant and Microbial Biology
Javier Sánchez-Martín: University of Zurich, Department of Plant and Microbial Biology
Helen Zbinden: University of Zurich, Department of Plant and Microbial Biology
Hana Šimková: Centre of the Region Hana for Biotechnological and Agricultural Research
Miroslava Karafiátová: Centre of the Region Hana for Biotechnological and Agricultural Research
Jaroslav Doležel: Centre of the Region Hana for Biotechnological and Agricultural Research
Julien Gronnier: University of Zurich, Department of Plant and Microbial Biology
Manuel Poretti: University of Zurich, Department of Plant and Microbial Biology
Gaétan Glauser: Université de Neuchâtel
Wangsheng Zhu: Max Planck Institute for Developmental Biology
Philipp Köster: University of Zurich, Department of Plant and Microbial Biology
Cyril Zipfel: University of Zurich, Department of Plant and Microbial Biology
Thomas Wicker: University of Zurich, Department of Plant and Microbial Biology
Simon G. Krattinger: King Abdullah University of Science and Technology (KAUST), Biological and Environmental Science and Engineering Division (BESE)
Beat Keller: University of Zurich, Department of Plant and Microbial Biology

Nature Communications, 2021, vol. 12, issue 1, 1-12

Abstract: Abstract Plasma membrane-associated and intracellular proteins and protein complexes play a pivotal role in pathogen recognition and disease resistance signaling in plants and animals. The two predominant protein families perceiving plant pathogens are receptor-like kinases and nucleotide binding-leucine-rich repeat receptors (NLR), which often confer race-specific resistance. Leaf rust is one of the most prevalent and most devastating wheat diseases. Here, we clone the race-specific leaf rust resistance gene Lr14a from hexaploid wheat. The cloning of Lr14a is aided by the recently published genome assembly of ArinaLrFor, an Lr14a-containing wheat line. Lr14a encodes a membrane-localized protein containing twelve ankyrin (ANK) repeats and structural similarities to Ca2+-permeable non-selective cation channels. Transcriptome analyses reveal an induction of genes associated with calcium ion binding in the presence of Lr14a. Haplotype analyses indicate that Lr14a-containing chromosome segments were introgressed multiple times into the bread wheat gene pool, but we find no variation in the Lr14a coding sequence itself. Our work demonstrates the involvement of an ANK-transmembrane (TM)-like type of gene family in race-specific disease resistance in wheat. This forms the basis to explore ANK-TM-like genes in disease resistance breeding.

Date: 2021
References: Add references at CitEc
Citations: View citations in EconPapers (3)

Downloads: (external link)
https://www.nature.com/articles/s41467-020-20777-x Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-020-20777-x

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-020-20777-x

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().