Ribosome-bound Get4/5 facilitates the capture of tail-anchored proteins by Sgt2 in yeast

Ying Zhang, Evelina Laurentiis, Katherine E. Bohnsack, Mascha Wahlig, Namit Ranjan, Simon Gruseck, Philipp Hackert, Tina Wölfle, Marina V. Rodnina, Blanche Schwappach () and Sabine Rospert ()
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Ying Zhang: University of Freiburg
Evelina Laurentiis: University Medical Center Göttingen
Katherine E. Bohnsack: University Medical Center Göttingen
Mascha Wahlig: University of Freiburg
Namit Ranjan: Max-Planck Institute for Biophysical Chemistry
Simon Gruseck: University of Freiburg
Philipp Hackert: University Medical Center Göttingen
Tina Wölfle: University of Freiburg
Marina V. Rodnina: Cluster of Excellence “Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells” (MBExC)
Blanche Schwappach: University Medical Center Göttingen
Sabine Rospert: University of Freiburg

Nature Communications, 2021, vol. 12, issue 1, 1-17

Abstract: Abstract The guided entry of tail-anchored proteins (GET) pathway assists in the posttranslational delivery of tail-anchored proteins, containing a single C-terminal transmembrane domain, to the ER. Here we uncover how the yeast GET pathway component Get4/5 facilitates capture of tail-anchored proteins by Sgt2, which interacts with tail-anchors and hands them over to the targeting component Get3. Get4/5 binds directly and with high affinity to ribosomes, positions Sgt2 close to the ribosomal tunnel exit, and facilitates the capture of tail-anchored proteins by Sgt2. The contact sites of Get4/5 on the ribosome overlap with those of SRP, the factor mediating cotranslational ER-targeting. Exposure of internal transmembrane domains at the tunnel exit induces high-affinity ribosome binding of SRP, which in turn prevents ribosome binding of Get4/5. In this way, the position of a transmembrane domain within nascent ER-targeted proteins mediates partitioning into either the GET or SRP pathway directly at the ribosomal tunnel exit.

Date: 2021
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-20981-3

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DOI: 10.1038/s41467-021-20981-3

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