A multiplex chemiluminescent immunoassay for serological profiling of COVID-19-positive symptomatic and asymptomatic patients

Allison N. Grossberg, Lilia A. Koza, Aurélie Ledreux, Chad Prusmack, Hari Krishnan Krishnamurthy, Vasanth Jayaraman, Ann-Charlotte Granholm and Daniel A. Linseman ()
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Allison N. Grossberg: Knoebel Institute for Healthy Aging, University of Denver
Lilia A. Koza: Knoebel Institute for Healthy Aging, University of Denver
Aurélie Ledreux: Knoebel Institute for Healthy Aging, University of Denver
Chad Prusmack: Resilience Code
Hari Krishnan Krishnamurthy: Vibrant Sciences LLC
Vasanth Jayaraman: Vibrant Sciences LLC
Ann-Charlotte Granholm: Knoebel Institute for Healthy Aging, University of Denver
Daniel A. Linseman: Knoebel Institute for Healthy Aging, University of Denver

Nature Communications, 2021, vol. 12, issue 1, 1-11

Abstract: Abstract The COVID-19 pandemic affects more than 81 million people worldwide with over 1.7 million deaths. As the population returns to work, it is critical to develop tests that reliably detect SARS-CoV-2-specific antibodies. Here we present results from a multiplex serology test for assessing the antibody responses to COVID-19. In an initial large cohort, this test shows greater than 99% agreement with COVID-19 PCR test. In a second outpatient cohort consisting of adults and children in Colorado, the IgG responses are more robust in positive/symptomatic participants than in positive/asymptomatic participants, the IgM responses in symptomatic participants are transient and largely fall below the detection limit 30 days after symptom onset, and the levels of IgA against SARS-CoV-2 receptor binding domain are significantly increased in participants with moderate-to-severe symptoms compared to those with mild-to-moderate symptoms or asymptomatic individuals. Our results thus provide insight into serology profiling and the immune response to COVID-19.

Date: 2021
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DOI: 10.1038/s41467-021-21040-7

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