Rapid electrochemical detection of coronavirus SARS-CoV-2

Thanyarat Chaibun, Jiratchaya Puenpa, Tatchanun Ngamdee, Nimaradee Boonapatcharoen, Pornpat Athamanolap, Anthony Peter O’Mullane, Sompong Vongpunsawad, Yong Poovorawan, Su Yin Lee and Benchaporn Lertanantawong ()
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Thanyarat Chaibun: Mahidol University
Jiratchaya Puenpa: Chulalongkorn University
Tatchanun Ngamdee: King Mongkut’s University of Technology Thonburi
Nimaradee Boonapatcharoen: King Mongkut’s University of Technology Thonburi
Pornpat Athamanolap: Mahidol University
Anthony Peter O’Mullane: Queensland University of Technology (QUT)
Sompong Vongpunsawad: Chulalongkorn University
Yong Poovorawan: Chulalongkorn University
Su Yin Lee: AIMST University
Benchaporn Lertanantawong: Mahidol University

Nature Communications, 2021, vol. 12, issue 1, 1-10

Abstract: Abstract Coronavirus disease 2019 (COVID-19) is a highly contagious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Diagnosis of COVID-19 depends on quantitative reverse transcription PCR (qRT-PCR), which is time-consuming and requires expensive instrumentation. Here, we report an ultrasensitive electrochemical biosensor based on isothermal rolling circle amplification (RCA) for rapid detection of SARS-CoV-2. The assay involves the hybridization of the RCA amplicons with probes that were functionalized with redox active labels that are detectable by an electrochemical biosensor. The one-step sandwich hybridization assay could detect as low as 1 copy/μL of N and S genes, in less than 2 h. Sensor evaluation with 106 clinical samples, including 41 SARS-CoV-2 positive and 9 samples positive for other respiratory viruses, gave a 100% concordance result with qRT-PCR, with complete correlation between the biosensor current signals and quantitation cycle (Cq) values. In summary, this biosensor could be used as an on-site, real-time diagnostic test for COVID-19.

Date: 2021
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-21121-7

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DOI: 10.1038/s41467-021-21121-7

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