Structural and biophysical correlation of anti-NANP antibodies with in vivo protection against P. falciparum

Tossapol Pholcharee, David Oyen, Yevel Flores-Garcia, Gonzalo Gonzalez-Paez, Zhen Han, Katherine L. Williams, Wayne Volkmuth, Daniel Emerling, Emily Locke, C. Richter King, Fidel Zavala and Ian A. Wilson ()
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Tossapol Pholcharee: The Scripps Research Institute
David Oyen: The Scripps Research Institute
Yevel Flores-Garcia: Johns Hopkins Bloomberg School of Public Health
Gonzalo Gonzalez-Paez: The Scripps Research Institute
Zhen Han: The Scripps Research Institute
Katherine L. Williams: Atreca Inc
Wayne Volkmuth: Atreca Inc
Daniel Emerling: Atreca Inc
Emily Locke: PATH’s Malaria Vaccine Initiative
C. Richter King: PATH’s Malaria Vaccine Initiative
Fidel Zavala: Johns Hopkins Bloomberg School of Public Health
Ian A. Wilson: The Scripps Research Institute

Nature Communications, 2021, vol. 12, issue 1, 1-14

Abstract: Abstract The most advanced P. falciparum circumsporozoite protein-based malaria vaccine, RTS,S/AS01 (RTS,S), confers partial protection but with antibody titers that wane relatively rapidly, highlighting the need to elicit more potent and durable antibody responses. Here, we elucidate crystal structures, binding affinities and kinetics, and in vivo protection of eight anti-NANP antibodies derived from an RTS,S phase 2a trial and encoded by three different heavy-chain germline genes. The structures reinforce the importance of homotypic Fab-Fab interactions in protective antibodies and the overwhelmingly dominant preference for a germline-encoded aromatic residue for recognition of the NANP motif. In this study, antibody apparent affinity correlates best with protection in an in vivo mouse model, with the more potent antibodies also recognizing epitopes with repeating secondary structural motifs of type I β- and Asn pseudo 310 turns; such insights can be incorporated into design of more effective immunogens and antibodies for passive immunization.

Date: 2021
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-21221-4

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DOI: 10.1038/s41467-021-21221-4

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