Addressable nanoantennas with cleared hotspots for single-molecule detection on a portable smartphone microscope

Kateryna Trofymchuk (), Viktorija Glembockyte (), Lennart Grabenhorst, Florian Steiner, Carolin Vietz, Cindy Close, Martina Pfeiffer, Lars Richter, Max L. Schütte, Florian Selbach, Renukka Yaadav, Jonas Zähringer, Qingshan Wei, Aydogan Ozcan, Birka Lalkens, Guillermo P. Acuna () and Philip Tinnefeld ()
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Kateryna Trofymchuk: Ludwig-Maximilians-Universität München
Viktorija Glembockyte: Ludwig-Maximilians-Universität München
Lennart Grabenhorst: Ludwig-Maximilians-Universität München
Florian Steiner: Ludwig-Maximilians-Universität München
Carolin Vietz: Technische Universität Braunschweig
Cindy Close: Ludwig-Maximilians-Universität München
Martina Pfeiffer: Ludwig-Maximilians-Universität München
Lars Richter: Technische Universität Braunschweig
Max L. Schütte: Technische Universität Braunschweig
Florian Selbach: Ludwig-Maximilians-Universität München
Renukka Yaadav: Ludwig-Maximilians-Universität München
Jonas Zähringer: Ludwig-Maximilians-Universität München
Qingshan Wei: North Carolina State University
Aydogan Ozcan: University of California
Birka Lalkens: Laboratory for Emerging Nanometrology LENA
Guillermo P. Acuna: Université de Fribourg - Faculté des Sciences et Médicine
Philip Tinnefeld: Ludwig-Maximilians-Universität München

Nature Communications, 2021, vol. 12, issue 1, 1-8

Abstract: Abstract The advent of highly sensitive photodetectors and the development of photostabilization strategies made detecting the fluorescence of single molecules a routine task in many labs around the world. However, to this day, this process requires cost-intensive optical instruments due to the truly nanoscopic signal of a single emitter. Simplifying single-molecule detection would enable many exciting applications, e.g., in point-of-care diagnostic settings, where costly equipment would be prohibitive. Here, we introduce addressable NanoAntennas with Cleared HOtSpots (NACHOS) that are scaffolded by DNA origami nanostructures and can be specifically tailored for the incorporation of bioassays. Single emitters placed in NACHOS emit up to 461-fold (average of 89 ± 7-fold) brighter enabling their detection with a customary smartphone camera and an 8-US-dollar objective lens. To prove the applicability of our system, we built a portable, battery-powered smartphone microscope and successfully carried out an exemplary single-molecule detection assay for DNA specific to antibiotic-resistant Klebsiella pneumonia on the road.

Date: 2021
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DOI: 10.1038/s41467-021-21238-9

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