Chromosomal coordination and differential structure of asynchronous replicating regions

Britny Blumenfeld, Hagit Masika, Marganit Farago, Yishai Yehuda, Lamia Halaseh, Oriya Vardi, Rachel Rapoport, Rena Levin-Klein, Howard Cedar (), Yehudit Bergman () and Itamar Simon ()
Additional contact information
Britny Blumenfeld: IMRIC, Hebrew University Medical School
Hagit Masika: Hebrew University Medical School
Marganit Farago: Hebrew University Medical School
Yishai Yehuda: IMRIC, Hebrew University Medical School
Lamia Halaseh: Hebrew University Medical School
Oriya Vardi: Hebrew University Medical School
Rachel Rapoport: IMRIC, Hebrew University Medical School
Rena Levin-Klein: Hebrew University Medical School
Howard Cedar: Hebrew University Medical School
Yehudit Bergman: Hebrew University Medical School
Itamar Simon: IMRIC, Hebrew University Medical School

Nature Communications, 2021, vol. 12, issue 1, 1-12

Abstract: Abstract Stochastic asynchronous replication timing (AS-RT) is a phenomenon in which the time of replication of each allele is different, and the identity of the early allele varies between cells. By taking advantage of stable clonal pre-B cell populations derived from C57BL6/Castaneous mice, we have mapped the genome-wide AS-RT loci, independently of genetic differences. These regions are characterized by differential chromatin accessibility, mono-allelic expression and include new gene families involved in specifying cell identity. By combining population level mapping with single cell FISH, our data reveal the existence of a novel regulatory program that coordinates a fixed relationship between AS-RT regions on any given chromosome, with some loci set to replicate in a parallel and others set in the anti-parallel orientation. Our results show that AS-RT is a highly regulated epigenetic mark established during early embryogenesis that may be used for facilitating the programming of mono-allelic choice throughout development.

Date: 2021
References: Add references at CitEc
Citations: View citations in EconPapers (2)

Downloads: (external link)
https://www.nature.com/articles/s41467-021-21348-4 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-21348-4

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-021-21348-4

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().