The TUTase URT1 connects decapping activators and prevents the accumulation of excessively deadenylated mRNAs to avoid siRNA biogenesis

Hélène Scheer, Caroline Almeida, Emilie Ferrier, Quentin Simonnot, Laure Poirier, David Pflieger, François M. Sement, Sandrine Koechler, Christina Piermaria, Paweł Krawczyk, Seweryn Mroczek, Johana Chicher, Lauriane Kuhn, Andrzej Dziembowski, Philippe Hammann, Hélène Zuber () and Dominique Gagliardi ()
Additional contact information
Hélène Scheer: Institut de biologie moléculaire des plantes, CNRS, Université de Strasbourg
Caroline Almeida: Institut de biologie moléculaire des plantes, CNRS, Université de Strasbourg
Emilie Ferrier: Institut de biologie moléculaire des plantes, CNRS, Université de Strasbourg
Quentin Simonnot: Institut de biologie moléculaire des plantes, CNRS, Université de Strasbourg
Laure Poirier: Institut de biologie moléculaire des plantes, CNRS, Université de Strasbourg
David Pflieger: Institut de biologie moléculaire des plantes, CNRS, Université de Strasbourg
François M. Sement: Institut de biologie moléculaire des plantes, CNRS, Université de Strasbourg
Sandrine Koechler: Institut de biologie moléculaire des plantes, CNRS, Université de Strasbourg
Christina Piermaria: Plateforme Protéomique Strasbourg Esplanade du CNRS, Université de Strasbourg
Paweł Krawczyk: International Institute of Molecular and Cell Biology
Seweryn Mroczek: International Institute of Molecular and Cell Biology
Johana Chicher: Plateforme Protéomique Strasbourg Esplanade du CNRS, Université de Strasbourg
Lauriane Kuhn: Plateforme Protéomique Strasbourg Esplanade du CNRS, Université de Strasbourg
Andrzej Dziembowski: International Institute of Molecular and Cell Biology
Philippe Hammann: Plateforme Protéomique Strasbourg Esplanade du CNRS, Université de Strasbourg
Hélène Zuber: Institut de biologie moléculaire des plantes, CNRS, Université de Strasbourg
Dominique Gagliardi: Institut de biologie moléculaire des plantes, CNRS, Université de Strasbourg

Nature Communications, 2021, vol. 12, issue 1, 1-17

Abstract: Abstract Uridylation is a widespread modification destabilizing eukaryotic mRNAs. Yet, molecular mechanisms underlying TUTase-mediated mRNA degradation remain mostly unresolved. Here, we report that the Arabidopsis TUTase URT1 participates in a molecular network connecting several translational repressors/decapping activators. URT1 directly interacts with DECAPPING 5 (DCP5), the Arabidopsis ortholog of human LSM14 and yeast Scd6, and this interaction connects URT1 to additional decay factors like DDX6/Dhh1-like RNA helicases. Nanopore direct RNA sequencing reveals a global role of URT1 in shaping poly(A) tail length, notably by preventing the accumulation of excessively deadenylated mRNAs. Based on in vitro and in planta data, we propose a model that explains how URT1 could reduce the accumulation of oligo(A)-tailed mRNAs both by favoring their degradation and because 3’ terminal uridines intrinsically hinder deadenylation. Importantly, preventing the accumulation of excessively deadenylated mRNAs avoids the biogenesis of illegitimate siRNAs that silence endogenous mRNAs and perturb Arabidopsis growth and development.

Date: 2021
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-21382-2

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DOI: 10.1038/s41467-021-21382-2

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