Point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP

Lukas Bokelmann, Olaf Nickel, Tomislav Maricic, Svante Pääbo, Matthias Meyer, Stephan Borte and Stephan Riesenberg ()
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Lukas Bokelmann: Max Planck Institute for Evolutionary Anthropology
Olaf Nickel: Hospital St. Georg
Tomislav Maricic: Max Planck Institute for Evolutionary Anthropology
Svante Pääbo: Max Planck Institute for Evolutionary Anthropology
Matthias Meyer: Max Planck Institute for Evolutionary Anthropology
Stephan Borte: Hospital St. Georg
Stephan Riesenberg: Max Planck Institute for Evolutionary Anthropology

Nature Communications, 2021, vol. 12, issue 1, 1-8

Abstract: Abstract Efforts to contain the spread of SARS-CoV-2 have spurred the need for reliable, rapid, and cost-effective diagnostic methods which can be applied to large numbers of people. However, current standard protocols for the detection of viral nucleic acids while sensitive, require a high level of automation and sophisticated laboratory equipment to achieve throughputs that allow whole communities to be tested on a regular basis. Here we present Cap-iLAMP (capture and improved loop-mediated isothermal amplification) which combines a hybridization capture-based RNA extraction of gargle lavage samples with an improved colorimetric RT-LAMP assay and smartphone-based color scoring. Cap-iLAMP is compatible with point-of-care testing and enables the detection of SARS-CoV-2 positive samples in less than one hour. In contrast to direct addition of the sample to improved LAMP (iLAMP), Cap-iLAMP prevents false positives and allows single positive samples to be detected in pools of 25 negative samples, reducing the reagent cost per test to ~1 Euro per individual.

Date: 2021
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DOI: 10.1038/s41467-021-21627-0

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