Global profiling of distinct cysteine redox forms reveals wide-ranging redox regulation in C. elegans

Jin Meng, Ling Fu, Keke Liu, Caiping Tian, Ziyun Wu, Youngeun Jung, Renan B. Ferreira, Kate S. Carroll, T. Keith Blackwell () and Jing Yang ()
Additional contact information
Jin Meng: Joslin Diabetes Center
Ling Fu: Beijing Institute of Lifeomics
Keke Liu: Beijing Institute of Lifeomics
Caiping Tian: Beijing Institute of Lifeomics
Ziyun Wu: Joslin Diabetes Center
Youngeun Jung: The Scripps Research Institute
Renan B. Ferreira: The Scripps Research Institute
Kate S. Carroll: The Scripps Research Institute
T. Keith Blackwell: Joslin Diabetes Center
Jing Yang: Beijing Institute of Lifeomics

Nature Communications, 2021, vol. 12, issue 1, 1-13

Abstract: Abstract Post-translational changes in the redox state of cysteine residues can rapidly and reversibly alter protein functions, thereby modulating biological processes. The nematode C. elegans is an ideal model organism for studying cysteine-mediated redox signaling at a network level. Here we present a comprehensive, quantitative, and site-specific profile of the intrinsic reactivity of the cysteinome in wild-type C. elegans. We also describe a global characterization of the C. elegans redoxome in which we measured changes in three major cysteine redox forms after H2O2 treatment. Our data revealed redox-sensitive events in translation, growth signaling, and stress response pathways, and identified redox-regulated cysteines that are important for signaling through the p38 MAP kinase (MAPK) pathway. Our in-depth proteomic dataset provides a molecular basis for understanding redox signaling in vivo, and will serve as a valuable and rich resource for the field of redox biology.

Date: 2021
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DOI: 10.1038/s41467-021-21686-3

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