Single-cell measurement of plasmid copy number and promoter activity

Bin Shao, Jayan Rammohan, Daniel A. Anderson, Nina Alperovich, David Ross and Christopher A. Voigt ()
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Bin Shao: Massachusetts Institute of Technology
Jayan Rammohan: National Institute of Standards and Technology
Daniel A. Anderson: Massachusetts Institute of Technology
Nina Alperovich: National Institute of Standards and Technology
David Ross: National Institute of Standards and Technology
Christopher A. Voigt: Massachusetts Institute of Technology

Nature Communications, 2021, vol. 12, issue 1, 1-9

Abstract: Abstract Accurate measurements of promoter activities are crucial for predictably building genetic systems. Here we report a method to simultaneously count plasmid DNA, RNA transcripts, and protein expression in single living bacteria. From these data, the activity of a promoter in units of RNAP/s can be inferred. This work facilitates the reporting of promoters in absolute units, the variability in their activity across a population, and their quantitative toll on cellular resources, all of which provide critical insights for cellular engineering.

Date: 2021
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-21734-y

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DOI: 10.1038/s41467-021-21734-y

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