Cell kinetics of auxin transport and activity in Arabidopsis root growth and skewing

Yangjie Hu, Moutasem Omary, Yun Hu, Ohad Doron, Lukas Hoermayer, Qingguo Chen, Or Megides, Ori Chekli, Zhaojun Ding, Jiří Friml, Yunde Zhao (), Ilan Tsarfaty () and Eilon Shani ()
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Yangjie Hu: Tel Aviv University
Moutasem Omary: Tel Aviv University
Yun Hu: University of California, San Diego
Ohad Doron: Tel Aviv University
Lukas Hoermayer: Institute of Science and Technology Austria
Qingguo Chen: University of California, San Diego
Or Megides: Tel Aviv University
Ori Chekli: Tel Aviv University
Zhaojun Ding: Shandong University
Jiří Friml: Institute of Science and Technology Austria
Yunde Zhao: University of California, San Diego
Ilan Tsarfaty: Tel Aviv University
Eilon Shani: Tel Aviv University

Nature Communications, 2021, vol. 12, issue 1, 1-13

Abstract: Abstract Auxin is a key regulator of plant growth and development. Local auxin biosynthesis and intercellular transport generates regional gradients in the root that are instructive for processes such as specification of developmental zones that maintain root growth and tropic responses. Here we present a toolbox to study auxin-mediated root development that features: (i) the ability to control auxin synthesis with high spatio-temporal resolution and (ii) single-cell nucleus tracking and morphokinetic analysis infrastructure. Integration of these two features enables cutting-edge analysis of root development at single-cell resolution based on morphokinetic parameters under normal growth conditions and during cell-type-specific induction of auxin biosynthesis. We show directional auxin flow in the root and refine the contributions of key players in this process. In addition, we determine the quantitative kinetics of Arabidopsis root meristem skewing, which depends on local auxin gradients but does not require PIN2 and AUX1 auxin transporter activities. Beyond the mechanistic insights into root development, the tools developed here will enable biologists to study kinetics and morphology of various critical processes at the single cell-level in whole organisms.

Date: 2021
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-21802-3

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DOI: 10.1038/s41467-021-21802-3

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