Programmable site-selective labeling of oligonucleotides based on carbene catalysis

Yang-Ha Lee, Eunsoo Yu and Cheol-Min Park ()
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Yang-Ha Lee: UNIST (Ulsan National Institute of Science & Technology)
Eunsoo Yu: UNIST (Ulsan National Institute of Science & Technology)
Cheol-Min Park: UNIST (Ulsan National Institute of Science & Technology)

Nature Communications, 2021, vol. 12, issue 1, 1-10

Abstract: Abstract Site-selective modification of oligonucleotides serves as an indispensable tool in many fields of research including research of fundamental biological processes, biotechnology, and nanotechnology. Here we report chemo- and regioselective modification of oligonucleotides based on rhodium(I)-carbene catalysis in a programmable fashion. Extensive screening identifies a rhodium(I)-catalyst that displays robust chemoselectivity toward base-unpaired guanosines in single and double-strand oligonucleotides with structurally complex secondary structures. Moreover, high regioselectivity among multiple guanosines in a substrate is achieved by introducing guanosine-bulge loops in a duplex. This approach allows the introduction of multiple unique functional handles in an iterative fashion, the utility of which is exemplified in DNA-protein cross-linking in cell lysates.

Date: 2021
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-21839-4

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DOI: 10.1038/s41467-021-21839-4

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