Trans-illumination intestine projection imaging of intestinal motility in mice

Depeng Wang, Huijuan Zhang, Tri Vu, Ye Zhan, Akash Malhotra, Pei Wang, Upendra Chitgupi, Aliza Rai, Sizhe Zhang, Lidai Wang, Jan D. Huizinga, Jonathan F. Lovell and Jun Xia ()
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Depeng Wang: University at Buffalo, State University of New York
Huijuan Zhang: University at Buffalo, State University of New York
Tri Vu: University at Buffalo, State University of New York
Ye Zhan: University at Buffalo, State University of New York
Akash Malhotra: City University of Hong Kong
Pei Wang: City University of Hong Kong
Upendra Chitgupi: University at Buffalo, State University of New York
Aliza Rai: City University of Hong Kong
Sizhe Zhang: University at Buffalo, State University of New York
Lidai Wang: City University of Hong Kong
Jan D. Huizinga: McMaster University
Jonathan F. Lovell: University at Buffalo, State University of New York
Jun Xia: University at Buffalo, State University of New York

Nature Communications, 2021, vol. 12, issue 1, 1-12

Abstract: Abstract Functional intestinal imaging holds importance for the diagnosis and evaluation of treatment of gastrointestinal diseases. Currently, preclinical imaging of intestinal motility in animal models is performed either invasively with excised intestines or noninvasively under anesthesia, and cannot reveal intestinal dynamics in the awake condition. Capitalizing on near-infrared optics and a high-absorbing contrast agent, we report the Trans-illumination Intestine Projection (TIP) imaging system for free-moving mice. After a complete system evaluation, we performed in vivo studies, and obtained peristalsis and segmentation motor patterns of free-moving mice. We show the in vivo typical segmentation motor pattern, that was previously shown in ex vivo studies to be controlled by intestinal pacemaker cells. We also show the effects of anesthesia on motor patterns, highlighting the possibility to study the role of the extrinsic nervous system in controlling motor patterns, which requires unanesthetized live animals. Combining with light-field technologies, we further demonstrated 3D imaging of intestine in vivo (3D-TIP). Importantly, the added depth information allows us to extract intestines located away from the abdominal wall, and to quantify intestinal motor patterns along different directions. The TIP system should open up avenues for functional imaging of the GI tract in conscious animals in natural physiological states.

Date: 2021
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DOI: 10.1038/s41467-021-21930-w

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