Multiplexed histology analyses for the phenotypic and spatial characterization of human innate lymphoid cells

Anna Pascual-Reguant, Ralf Köhler, Ronja Mothes, Sandy Bauherr, Daniela C. Hernández, Ralf Uecker, Karolin Holzwarth, Katja Kotsch, Maximilian Seidl, Lars Philipsen, Werner Müller, Chiara Romagnani, Raluca Niesner and Anja E. Hauser ()
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Anna Pascual-Reguant: Department of Rheumatology and Clinical Immunology
Ralf Köhler: Deutsches Rheuma-Forschungszentrum (DRFZ), a Leibniz Institute
Ronja Mothes: Department of Rheumatology and Clinical Immunology
Sandy Bauherr: Deutsches Rheuma-Forschungszentrum (DRFZ), a Leibniz Institute
Daniela C. Hernández: Deutsches Rheuma-Forschungszentrum (DRFZ), a Leibniz Institute
Ralf Uecker: Department of Rheumatology and Clinical Immunology
Karolin Holzwarth: Deutsches Rheuma-Forschungszentrum (DRFZ), a Leibniz Institute
Katja Kotsch: Charité - Universitätsmedizin Berlin, Department for General, Visceral and Vascular Surgery
Maximilian Seidl: Medical Center—University of Freiburg
Lars Philipsen: Institute of Molecular and Clinical Immunology, Medical Center
Werner Müller: Miltenyi Biotec B.V. & Co. KG
Chiara Romagnani: Deutsches Rheuma-Forschungszentrum (DRFZ), a Leibniz Institute
Raluca Niesner: Deutsches Rheuma-Forschungszentrum (DRFZ), a Leibniz Institute
Anja E. Hauser: Department of Rheumatology and Clinical Immunology

Nature Communications, 2021, vol. 12, issue 1, 1-15

Abstract: Abstract Innate lymphoid cells (ILCs) emerge in the last few years as important regulators of immune responses and biological processes. Although ILCs are mainly known as tissue-resident cells, their precise localization and interactions with the microenvironment are still unclear. Here we combine a multiplexed immunofluorescence technique and a customized computational, open-source analysis pipeline to unambiguously identify CD127+ ILCs in situ and characterize these cells and their microenvironments. Moreover, we reveal the transcription factor IRF4 as a marker for tonsillar ILC3, and identify conserved stromal landmarks characteristic for ILC localization. We also show that CD127+ ILCs share tissue niches with plasma cells in the tonsil. Our works thus provide a platform for multiparametric histological analysis of ILCs to improve our understanding of ILC biology.

Date: 2021
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-21994-8

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DOI: 10.1038/s41467-021-21994-8

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