Bacterial cyclic diguanylate signaling networks sense temperature

Henrik Almblad, Trevor E. Randall, Fanny Liu, Katherine Leblanc, Ryan A. Groves, Weerayuth Kittichotirat, Geoffrey L. Winsor, Nicolas Fournier, Emily Au, Julie Groizeleau, Jacquelyn D. Rich, Yuefei Lou, Elise Granton, Laura K. Jennings, Larissa A. Singletary, Tara M. L. Winstone, Nathan M. Good, Roger E. Bumgarner, Michael F. Hynes, Manu Singh, Maria Silvina Stietz, Fiona S. L. Brinkman, Ayush Kumar, Ann Karen Cornelia Brassinga, Matthew R. Parsek, Boo Shan Tseng, Ian A. Lewis, Bryan G. Yipp, Justin L. MacCallum and Joe Jonathan Harrison ()
Additional contact information
Henrik Almblad: University of Calgary
Trevor E. Randall: University of Calgary
Fanny Liu: University of Calgary
Katherine Leblanc: University of Calgary
Ryan A. Groves: University of Calgary
Weerayuth Kittichotirat: Pilot Plant Development and Training Institute, King Mongkut’s University of Technology Thonburi
Geoffrey L. Winsor: Simon Fraser University
Nicolas Fournier: University of Calgary
Emily Au: University of Calgary
Julie Groizeleau: University of Calgary
Jacquelyn D. Rich: University of Calgary
Yuefei Lou: University of Calgary
Elise Granton: University of Calgary
Laura K. Jennings: University of Washington
Larissa A. Singletary: University of Washington
Tara M. L. Winstone: University of Calgary
Nathan M. Good: University of Washington
Roger E. Bumgarner: University of Washington
Michael F. Hynes: University of Calgary
Manu Singh: University of Manitoba
Maria Silvina Stietz: University of Calgary
Fiona S. L. Brinkman: Simon Fraser University
Ayush Kumar: University of Manitoba
Ann Karen Cornelia Brassinga: University of Manitoba
Matthew R. Parsek: University of Washington
Boo Shan Tseng: University of Nevada Las Vegas
Ian A. Lewis: University of Calgary
Bryan G. Yipp: University of Calgary
Justin L. MacCallum: University of Calgary
Joe Jonathan Harrison: University of Calgary

Nature Communications, 2021, vol. 12, issue 1, 1-14

Abstract: Abstract Many bacteria use the second messenger cyclic diguanylate (c-di-GMP) to control motility, biofilm production and virulence. Here, we identify a thermosensory diguanylate cyclase (TdcA) that modulates temperature-dependent motility, biofilm development and virulence in the opportunistic pathogen Pseudomonas aeruginosa. TdcA synthesizes c-di-GMP with catalytic rates that increase more than a hundred-fold over a ten-degree Celsius change. Analyses using protein chimeras indicate that heat-sensing is mediated by a thermosensitive Per-Arnt-SIM (PAS) domain. TdcA homologs are widespread in sequence databases, and a distantly related, heterologously expressed homolog from the Betaproteobacteria order Gallionellales also displayed thermosensitive diguanylate cyclase activity. We propose, therefore, that thermotransduction is a conserved function of c-di-GMP signaling networks, and that thermosensitive catalysis of a second messenger constitutes a mechanism for thermal sensing in bacteria.

Date: 2021
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-22176-2

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DOI: 10.1038/s41467-021-22176-2

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