Widespread reorganisation of pluripotent factor binding and gene regulatory interactions between human pluripotent states
Peter Chovanec,
Amanda J. Collier,
Christel Krueger,
Csilla Várnai,
Claudia I. Semprich,
Stefan Schoenfelder,
Anne E. Corcoran and
Peter J. Rugg-Gunn ()
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Peter Chovanec: Babraham Institute
Amanda J. Collier: Babraham Institute
Christel Krueger: Babraham Institute
Csilla Várnai: Babraham Institute
Claudia I. Semprich: Babraham Institute
Stefan Schoenfelder: Babraham Institute
Anne E. Corcoran: Babraham Institute
Peter J. Rugg-Gunn: Babraham Institute
Nature Communications, 2021, vol. 12, issue 1, 1-18
Abstract:
Abstract The transition from naive to primed pluripotency is accompanied by an extensive reorganisation of transcriptional and epigenetic programmes. However, the role of transcriptional enhancers and three-dimensional chromatin organisation in coordinating these developmental programmes remains incompletely understood. Here, we generate a high-resolution atlas of gene regulatory interactions, chromatin profiles and transcription factor occupancy in naive and primed human pluripotent stem cells, and develop a network-graph approach to examine the atlas at multiple spatial scales. We uncover highly connected promoter hubs that change substantially in interaction frequency and in transcriptional co-regulation between pluripotent states. Small hubs frequently merge to form larger networks in primed cells, often linked by newly-formed Polycomb-associated interactions. We identify widespread state-specific differences in enhancer activity and interactivity that correspond with an extensive reconfiguration of OCT4, SOX2 and NANOG binding and target gene expression. These findings provide multilayered insights into the chromatin-based gene regulatory control of human pluripotent states.
Date: 2021
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-22201-4
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DOI: 10.1038/s41467-021-22201-4
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