Cargo sorting zones in the trans-Golgi network visualized by super-resolution confocal live imaging microscopy in plants

Yutaro Shimizu, Junpei Takagi, Emi Ito, Yoko Ito, Kazuo Ebine, Yamato Komatsu, Yumi Goto, Mayuko Sato, Kiminori Toyooka, Takashi Ueda, Kazuo Kurokawa, Tomohiro Uemura () and Akihiko Nakano ()
Additional contact information
Yutaro Shimizu: RIKEN Center for Advanced Photonics, Wako
Junpei Takagi: Konan University, Kobe
Emi Ito: Ochanomizu University
Yoko Ito: RIKEN Center for Advanced Photonics, Wako
Kazuo Ebine: National Institute for Basic Biology
Yamato Komatsu: The University of Tokyo
Yumi Goto: Mass Spectrometry and Microscopy Unit, RIKEN Center for Sustainable Resource Science
Mayuko Sato: Mass Spectrometry and Microscopy Unit, RIKEN Center for Sustainable Resource Science
Kiminori Toyooka: Mass Spectrometry and Microscopy Unit, RIKEN Center for Sustainable Resource Science
Takashi Ueda: National Institute for Basic Biology
Kazuo Kurokawa: RIKEN Center for Advanced Photonics, Wako
Tomohiro Uemura: Ochanomizu University
Akihiko Nakano: RIKEN Center for Advanced Photonics, Wako

Nature Communications, 2021, vol. 12, issue 1, 1-14

Abstract: Abstract The trans-Golgi network (TGN) has been known as a key platform to sort and transport proteins to their final destinations in post-Golgi membrane trafficking. However, how the TGN sorts proteins with different destinies still remains elusive. Here, we examined 3D localization and 4D dynamics of TGN-localized proteins of Arabidopsis thaliana that are involved in either secretory or vacuolar trafficking from the TGN, by a multicolor high-speed and high-resolution spinning-disk confocal microscopy approach that we developed. We demonstrate that TGN-localized proteins exhibit spatially and temporally distinct distribution. VAMP721 (R-SNARE), AP (adaptor protein complex)−1, and clathrin which are involved in secretory trafficking compose an exclusive subregion, whereas VAMP727 (R-SNARE) and AP-4 involved in vacuolar trafficking compose another subregion on the same TGN. Based on these findings, we propose that the single TGN has at least two subregions, or “zones”, responsible for distinct cargo sorting: the secretory-trafficking zone and the vacuolar-trafficking zone.

Date: 2021
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-021-22267-0 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-22267-0

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-021-22267-0

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().